数据来源:日本某药企(PJ-TA-JP-01) 使用芯片:生物活性分子阵列 服务内容:筛选靶标蛋白,寻找所有药物分子与11种靶标蛋白的结合信息,提供准确动力学,亲和力常数 方法:多浓度梯度拟合,多重3倍SD筛选 时间:6小时 结果:客户提供阴阳性对照全部验证;不同缓冲液下的实验,重复率高,满足预期;热图提供信息丰富可靠,为分子设计与靶标蛋白提供快速,精确的解决方案。 已经发表或正在发表文章:企业研究内容,暂不公开发表。 案例2: 激酶抑制剂库 产品描述及特点:芯片上打印了355多种激酶抑制剂阵列,涉及与其相互作用的RTKs, PI3K, Aurora激酶, CDK和MEK等激酶,为客户高通量,高内涵筛选服务 产品用途:筛选激酶抑制新靶标(老药新用);激酶抑制剂活性盲筛;建立靶标蛋白或激酶抑制剂的指纹数据库;
数据来源:约翰·霍普金斯大学(PJ-LJ-USA-01) 使用芯片:激酶抑制剂库 服务内容:筛选与FKBP12及其6个突变体相互作用小分子,提供准确动力学,亲和力常数 方法:多浓度梯度拟合,多重3倍SD筛选,交互印证实验 结果:通过固定小分子筛选蛋白及固定蛋白筛选小分子的交互实验,成功筛选出至少2个与FKBP12突变体相互作用小分子,得到了其动力学,亲和力常数,为后期生物实验及临床实验提供可靠的初步筛选信息,大节省了初筛时间与成本。
用户文献: Enhancement of the antagonistic potency of transforming growth factor-beta receptor extracellular domains by coiled coil-induced homo- and heterodimerization. De Crescenzo G, Pham PL, Durocher Y, Chao H, O'Connor-McCourt MD. J Biol Chem. 2004 Jun 18;279(25):26013-8. Waveguide coupled surface plasmon resonance imaging measurement and high-throughput analysis of bio-interaction. Lusheng Song, Zhiyou Wang, Dasu Zhou, Zheng Zheng, Amita Nand, Shaopeng Li, Bihong Guo, Yanmei Wang, Zhiqiang Cheng, Wenfei Zhou, Jinsong Zhu., Biosensors and Bioelectronics Poly(acrylic acid) Brushes Pattern as 3D functional surface for biosensor applications. YanMei Wang*, Yi Cui*, ZhiQiang Cheng, LuSheng Song, ZhiYou Wang, BaoHang Han, JinSong Zhu.*Applied surface science, 2013 Feburary 1,vol266 (313-318) Stable and Sensitive Silver Surface Plasmon Resonance Imaging Sensor Using Trilayered Metallic Structures. Zhiyou Wang, Zhiqiang Cheng, Vikramjeet Singh, Zheng Zheng, Yanmei Wang, Shaopeng Li, Lusheng Song, and Jinsong Zhu. Analytical Chemistry 2014 86 (3), 1430-1436 Using Self-Polymerized Dopamine to Modify the Antifouling Property of Oligo(ethylene glycol) Self-Assembled Monolayers and Its Application in Cell Patterning. Kang Sun, Lusheng Song, Yunyan Xie, Dingbin Liu, Dong Wang, Zhuo Wang, Wanshun Ma, Jinsong Zhu, and Xingyu Jiang. Langmuir 2011 27 (10), 5709-5712 Quantitative Liver-Specific Protein Fingerprint in Blood: A Signature for Hepatotoxicity. Zhiyuan Hu, Christopher Lausted, Hyuntae Yoo, Xiaowei Yan, Amy Brightman, Jiankui Chen, Weizhi Wang , Xiangli Bu1 and Leroy Hood.Theranostics 2014, Vol. 4, Issue 2. 215-228 Bimodal Imprint Chips for Peptide Screening: Integration of High-Throughput Sequencing by MS and Affinity Analyses by Surface Plasmon Resonance Imaging. Weizhi Wang, Menglin Li, Zewen Wei, Zihua Wang, Xiangli Bu, Wenjia Lai, Shu Yang, He Gong, Hui Zheng,Yuqiao Wang, Ying Liu,§ Qin Li, Qiaojun Fang, and Zhiyuan Hu. Analytical Chemistry. 2014, 86, 3703?3707 |