截留分子量为3K,10K,30K,50K,100K几种。每种包装规格有8个 24个 96个。
- High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
- High retentate recovery of >90%
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
- Heat-sealed membrane minimizes downstream extractables
- 100% integrity tested for reliable performance
- Convenient sample monitoring with translucent housing and volume gradations
- Direct pipettor sample access eliminates processing step to recover concentrate
- High concentration factors of 80–100X
Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting, buffer exchange and protein dialysis
- Size exclusion and protein fractionation
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Typical Spin Times to Filtrate Volume
Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery (return to top)
Membrane
NMWL, kDa |
Protein Solute |
Protein Retentate
Volume, mL |
Protein Retentate
Recovery, % |
3 |
Cytochrome c, 12.4 kDa
(0.25 mg/mL) |
0.294 |
97.5 |
10 |
Cytochrome c, 12.4 kDa
(0.25 mg/mL) |
0.24 |
93.03 |
30 |
BSA, 67 kDa
(1 mg/mL) |
0.30 |
97.94 |
50 |
BSA, 67 kDa
(1 mg/mL) |
0.17 |
93.32 |
100 |
IgG, 156 kDa
(1 mg/mL) |
0.28 |
89.03 |
Specifications (return to top)
Volumes |
Maximum Initial Sample Volume, mL* |
15 |
Typical Final Concentrate Volume, µL |
200 |
Centrifugal Force |
Swinging bucket rotor: 4,000 x g; Fixed angle rotor (35°): 5,000 x g |
Materials |
Membrane |
Ultracel regenerated cellulose |
Device |
Housing: Styrene/butadiene
Tube: Polypropylene
Cap and liner: Polyethylene |
Dimensions |
Active Membrane Area, cm2 |
7.6 |
Diameter, mm |
29.7 |
Filtrate tube or vial (centrifuge tube) |
Length, mm (with cap and device) |
122 |
Filter unit |
Diameter, mm |
29.6 |
Length, mm |
72.0 |
Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane is CE marked.
目录编号 |
产品名称 |
NMWL,kDa |
数量/包装 |
UFC900308 |
装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管 |
3 |
8 |
UFC900324 |
装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管 |
3 |
24 |
UFC900396 |
装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管 |
3 |
96 |
UFC901008 |
装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管 |
10 |
8 |
UFC901024 |
装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管 |
10 |
24 |
UFC901096 |
装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管 |
10 |
96 |
UFC903008 |
装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管 |
30 |
8 |
UFC903024 |
装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管 |
30 |
24 |
UFC903096 |
装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管 |
30 |
96 |
UFC905008 |
装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管 |
50 |
8 |
UFC905024 |
装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管 |
50 |
24 |
UFC905096 |
装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管 |
50 |
96 |
UFC910008 |
装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管 |
100 |
8 |
UFC910024 |
装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管 |
100 |
24 |
UFC910096 |
装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管 |
100 |
96 |
|