产品介绍
The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in fifteen minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 2...
Reverse Transcription System( 逆转录系统) 可在15 分钟内高效地将RNA 逆转录为cDNA。该系统合成的cDNA 可直接使用Promega 公司的GoTaq? DNA 聚合酶进行多重PCR 反应。AMV 逆转录酶可从总RNA 或poly(A)+ RNA 合成单链cDNA,并且系统提供Oligo(dT)15 和随机引物,可从任何来源的RNA 中进行cDNA 合成。该系统的试剂足够进行100次cDNA 合成反应,每次反应使用1μg RNA模板。每次合成的cDNA产物能够用于多至20 次的PCR 反应。另外系统还提供了一个1.2kb 多聚腺苷酸化RNA 转录子作为cDNA合成反应的阳性对照模板。