Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Catalogue Number
539480
Brand Family
Calbiochem?
References
References
Watazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81. Lebherz, H.G., et al. 1986. Biochem. J.233, 51. Ebeling, W., et al. 1974. Eur. J. Biochem.47, 91.
Product Information
CAS number
39450-01-6
Activity
≥30 mAnsonU/mg dry weight
Unit of Definition
One mAnson unit is defined as the amount of enzyme that will liberate 1.0 μmol of Folin-positive amino acids (calculated as tyrosine) per min at 25°C, pH 7.5.
EC number
3.4.21.14
Form
White lyophilized solid
Formulation
Lyophilized from calcium acetate
Biological Information
Specific Activity
≥40 mAnsonU/mg protein
Physicochemical Information
Contaminants
DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.