来宝网移动站

重组人表皮生长因子85折促销

来宝网 2009/11/8点击2501次

Recombinant Human Epidermal Growth Factor (EGF)

目录号 规格 价格
C029A 10μg 120元
C029B 50μg 360元
C029C 1mg 2200元

*大包装请询价

Background:
Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF is a small 53 amino acid residue long protein that contains three disulfide briges.
Description:
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Quality Control:
Biological activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0x 105 IU/ mg.
Purity: Greater than 95% as determined by
        (a) Analysis by SEC-HPLC.
        (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Molecular weight: 6.0KD+/-10% determined by reduced Tricine-SDS-PAGE.
Isoelectric Point: the main zone between 4.0～5.0 analysis by IEF.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu.
Endotoxin: Less than 0.1ng/μg (1 IEU/μg) determined by LAL test.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Storage:

Lyophilized rHuEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuEGF should be stored at 4°C between 2-7 days and for future use below -18°C. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).
 Please avoid freeze-thaw cycles.
Reconstitution:
It is recommended to reconstitute the lyophilized rHuEGF in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
For laboratory in vitro research use only.

推荐仪器
  • *
  • *
  • *
  • *