来宝网 2016/5/13点击1099次
1. Background
Vimentin: The type III intermediate filament protein Vimentin is the major cytoskeletal component of mesenchymal cells. It is expressed in a highly developmentally-regulated fashion. Therefore vimentin has become an important biomarker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression, highly dynamic cellular processes.
Chromobodies：ChromoTek has developed Chromobodies®, which visualize endogenous proteins in live cells and at real time, to overcome limitations of the current protein visualization methods antibody staining and fluorescent fusion protein expression:
• Antibodies are valuable tools for the visualization of endogenous cellular components, however this procedure requires cell fixation restricting their applicability to end-point measurements. The traditional antibodies’ use in living cells even as fragments is limited because of the inefficient folding and assembly of their variable heavy and light chains.
• Fluorescent fusion proteins can easily be overexpressed in cells and provide information on protein localization and dynamics in living cells, but endogenous proteins remain invisible. Fusion protein (over-) expression may also be cytotoxic and may introduce artificial effects.
2. Product Description
The Vimentin Chromobody is a functional intracellular antibody (so called “nanobody”/VHH derived from Alpaca), which is genetically fused to Green Fluorescent Protein (TagGFP2 only!) for real time analysis of endogenous vimentin in live cells. The Vimentin Chromobody is available as plasmid for mammalian cell transfection. It is sold as plasmid in quantities of 20 µg using product code “vcg” at standard Chromobody pricing. The Vimentin Chromobody-TagGFP plasmid is shipped at room temperature and is accompanied by a vector map. Lentiviral particles and stable cell lines are available on request as custom services. Please inquire. Note: A genetic fusion to tagRFP is not available.
3. Positioning Statement
The Vimentin Chromobody is a proprietary, ground breaking tool for detection of endogenous vimentin in real-time in live cells. It is designed to overcome limitations of protein analysis in cells using antibody staining and fusion protein expression.
4. Value Proposition
The Vimentin Chromobody is a unique tool for visualization of endogenous vimentin in real-time in live cells. It has been carefully tested to not interfere with the biological functions of vimentin.
• Dynamic live-cell analysis, (but also end-point assays if needed)*
• Fast no-wash assay (compared to antibody staining)*
• Visualization of endogenous vimentin
• No vimentin overexpression, which would in fact induce EMT phenotype
• No cytotoxicity and artificial effects
*Main advantages of the Vimentin Chromobody.
5. Key Words
Vimentin, epithelial (to) mesenchymal transition, EMT, biomarker, intracellular antibody/nanobody, Chromobody, intrabody, live cell imaging, real-time visualization, cancer, oncology, small molecule screens.
订货信息:
| 货号 | 品名 | 规格 |
| vcg | Vimentin-Chromobody® plasmid (TagGFP) | 20 µg |
| vba488-10 | Vimentin-Booster_Atto488 | 10µl |
| vba488-100 | Vimentin-Booster_Atto488 | 100µl |
