产品报价：4800元
更新时间：2023/3/20 15:11:05
产地：湖北
品牌：NewEast Biosciences
型号：EGFR(G863D)
厂商性质： 生产型,服务型,
公司名称： 武汉费斯德生物科技有限公司
黎敏 : (18162422833) (027-63497286)
（联系我时，请说明是在来宝网上看到的，谢谢！）| 货号: 26119 |
| 基因符号: EGFR/HER1/ERBB1 |
| 描述: Anti-EGFR(G863D) Mouse Monoclonal Antibody |
| 背景介绍: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| 免疫原: A synthetic peptide from the internal region of EGFR which includes the mutation of G863D, human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 1000-1: 5000 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes EGFR(G863D), but not wild type EGFR from vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant EGFR(G863D) and wildtype proteins. Purified His-tagged EGFR(G863D) protein (amino acid 702-987) (Cat. #10222) and corresponding wild-type protein (Cat. #10210) were blotted with anti-EGFR(G863D) mouse monoclonal antibody (Cat. #26119). Proteins loading were blotted with anti-His-tag monoclonal antibody (Cat. #28004). 免疫荧光:
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(G863D) plasmid (right column), then fixed and stained with anti-EGFR(G863D) monoclonal antibody (Cat. #26119). |
