位于慕尼黑附近Penzberg的罗氏工厂是全球最大 的生化试剂、诊断试剂和药品的生产基地之一。出产的产品除罗氏自用外，还作为原材料提供给其他的生产厂家。
根据客户的要求，产品可在GMP和GLP标准下生产，并符合FDA，USDA和BGA的规定。厂房通过ISO 9001论证。
一. PCR级重组蛋白酶K
性能优势：1.GMP级别；2.可在室温下长期稳定保存；3.有冻干粉和液态产品供选择。
可根据客户需求提供不同包装的工业原料，价格实惠。
主要通用包装有：03 508 838 103 100 mg（常备现货） 05 963 117 103 5 g
Proteinase K, recombinant, PCR Grade
from Titrirachium album, expressed in Pichia pastoris,
lyophilizate
Application
Proteinase K, recombinant, PCR Grade, digests native proteins very efficiently.
This enzyme can be used to rapidly inactivate endogenous RNases and
DNases during nucleic acid isolation. Proteinase K is particularly suited for the
isolation of native RNA and DNA from tissues and cell lines.
The enzyme promotes cell lysis by activating a bacterial autolytic factor.
Proteinase K is also used for:
■ Analysis of membrane structures by modifying proteins and glycoproteins
on cell surfaces
■ Removal of cellular debris during the preparation of colony lifts
■ Treatment of tissue sections to ensure efficient probe infiltration during in
situ hybridization
Properties
Nomenclature: Proteinase K
Molecular weight: 28.8 kD
pH optimum: 7.5-10.5
Inhibitors: Proteinase K is inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl
fluoride (PMSF) and is also totally inactivated by mercuric
ions. Pefabloc? SC and Pefabloc? PLUS are specific, irreversible and nontoxic
inhibitors.
Specificity: Proteinase K is one of the most active endopeptidases known and
does not exhibit any pronounced cleavage specificity. Activity can be stimulated
by addition of denaturing agents (SDS and urea).
Specification
Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/ml)
Volume activity (+37°C, hemoglobin): ≥24 U/mg lyophilizate
Specific activity (+37°C, hemoglobin): ≥30 U/mg protein
Unit definition (hemoglobin): One unit is the enzyme activity which releases
folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1
minute under the test conditions.
Volume activity (+25°C, Chromozym): ≥2 U/mg lyophilizate
Specific activity (+25°C, Chromozym): ≥2.5 U/mg protein
Unit definition (Chromozym): One unit is the enzyme activity which cleaves at
+25°C in 1 minute 18 mmol Chromozym TRY.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg
after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours
incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours
incubation at +37°C.
DNA (Threshold): ≤10 pg/mg enzyme
Bioburden: ≤125 CFU/g
Stability: At +2 to +8°C within specification range for 18 months.
