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现在位置首页>实验室原料试剂耗材>化学试剂>材料科学>MQ7.101 anti-T. cruzi (clone 8E81F4)
MQ7.101	anti-T. cruzi (clone 8E81F4)
MQ7.101	anti-T. cruzi (clone 8E81F4)
  • MQ7.101	anti-T. cruzi (clone 8E81F4)

MQ7.101 anti-T. cruzi (clone 8E81F4)

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:ImmunoPrecise

号:MQ7.101

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词: mouse anti-Trypanosoma cruzi a   anti-T. cruzi (clone 8E81F4)   MQ7.101   MQ7.101 anti-T. cruzi (clone 8   Mouse IgG  

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品名


单克隆小鼠


抗克鲁齐锥虫,


免疫球蛋白,克隆


8E81F4型


货号。


MQ 7.101-100标准


批号。


15071号


数量


100微克


版本:2015年4月22日


预期用途


本产品仅供研究使用。不用于诊断


或治疗程序。


该产品经测试可用于酶联免疫吸附


ELISA法和免疫荧光法。


提供试剂


抗体已在10毫米铵中冻干


碳酸氢盐缓冲液。


同型


小鼠免疫球蛋白


免疫原


P015肽;来自核糖体的C末端肽


克鲁兹锥虫蛋白质。


特异性


特异性已在酶联免疫吸附试验(图1)和


免疫荧光(图2)。


纯度


纯化蛋白A。


注意事项


一。对于专业用户。


2。与任何来自生物源的产品一样


应使用处理程序。


三。该产品可用于不同的技术和


结合不同的样品类型和材料,


因此,每个实验室应验证


应用测试系统。


抗体的制备


?推荐抗体浓度:0.5 mg/ml。


?推荐溶剂:100 mM PBS或Tris HCl,pH 7.0。


?建议添加叠氮化钠(高达0.05%)


用于长期储存。


?对于0.5 mg/ml抗体浓度,溶于200微升


缓冲区。注意:小心打开小瓶,因为抗体位于


真空吸尘器。


存储说明


对于长期储存,将冻干批次保持在-20°C。


溶解后,在2-8°C下储存。对于长期储存,添加钠


叠氮化物含量为0.05%。


应用指南


酶联免疫吸附试验:0.04–10微克/毫升


免疫荧光:1,5微克/毫升


除非已经确定了实际测试系统的稳定性,


建议在使用前立即稀释产品。


关联


T、 克鲁齐是一种原生动物寄生虫。一旦感染,这种寄生虫就会引起


查加斯病,一种热带疾病,由三爪亚目咬伤引起,


对某些病人来说,这可能会导致炉缸衰竭。患者


慢性查加斯


疾病产生抗P2β和P0的抗体(两个核糖体


cruzi的蛋白质),与心脏受体发生交叉反应。


0个


0,2个


0,4个


0,6个


0,8个


1个


1,2个


T、 克鲁兹细胞


裂解物


P015马力


牛血清白蛋白


H26R肽M2肽


外径(450nm)


图1:抗T.cruzi免疫球蛋白克隆8E81F4的特异性,测定


用ELISA法。抗体在含有0.05%吐温-20和1%牛血清白蛋白的PBS中稀释至75微克/毫升


在T.cruzi细胞裂解物上进行测试,P015肽(来自


克鲁兹T.cruzi的核糖体P0蛋白),


H26R蛋白(B1受体;阴性对照)和M2肽(毒蕈碱


受体;阴性对照)。


图2:抗T.cruzi免疫球蛋白克隆8E81F4的特异性,


用免疫荧光法检测克氏锥虫。细胞


与MQ7.101(1,5微克/毫升)和抗-Tc13固定、渗透和孵育


(1/500;识别一种克鲁兹T.cruzi膜蛋白)。抗体的结合是


用抗鼠IgG-Alexa 488(1/1000)和抗兔IgG-Cy3(1/200)检测。


确认


K、 A.戈麦斯和L.M.塔索


恰加斯病分子生物学实验室(LaBMECh),


研究所


分子生物学研究


阿根廷布宜诺斯艾利斯。




Product Name

Monoclonal Mouse

Anti-Trypanosoma cruzi,

Immunoglobulin, clone

8E81F4

CAT No.

MQ 7.101-100

LOT No.

15071

Quantity

100 μg

Edition: April 22nd, 2015

Intended use

This product is for research use only. NOT for use in diagnostic

or therapeutic procedures.

This product is tested for use in enzyme-linked immunosorbent

assay (ELISA) and immunofluorescence.

Reagent provided

The antibody has been lyophilized in a 10 mM ammonium

bicarbonate buffer.

Isotype

Mouse IgG

Immunogen

P015 peptide; C-terminal peptide from the ribosomal P0

protein of Trypanosoma cruzi (T. cruzi).

Specificity

Specificity has been tested in ELISA (figure 1) and

immunofluorescence (figure 2).

Purity

Protein A purified.

Precautions

1. For professional users.

2. As with any product derived from biological sources, proper

handling procedures should be used.

3. The product may be used in different techniques and in

combination with different sample types and materials,

therefore each individual laboratory should validate the

applied test system.

Preparation of the antibody

? Recommended antibody concentration: 0.5 mg/ml.

? Recommended solvent: 100 mM PBS or Tris-HCl, pH 7.0.

? Additional sodium azide (up to 0.05%) is recommended

for prolonged storage.

? For a 0.5 mg/ml antibody concentration, dissolve in 200 μl

buffer. NOTE: Be careful opening the vial since the antibody resides in

a vacuum.

Storage instructions

For long-term storage keep lyophilized batch at -20°C.

After dissolving, store at 2-8°C. For prolonged storage add sodium

azide to 0.05%.

Application guidelines

ELISA: 0.04 – 10 μg/ml

Immunofluorescence: 1,5 μg/ml

Unless the stability in the actual test system has been established,

it is recommended to dilute the product immediately before use.

Relevance

T. cruzi is a protozoan parasite. Upon infection, this parasite causes

Chagas disease, a tropical disease caused by a bite by Triatominae,

which, in some patients, might result in hearth failure. Patients

with chronic Chagas

disease develop antibodies against P2β and P0 (two ribosomal

proteins of T. cruzi), which cross-react with cardiac receptor.

0

0,2

0,4

0,6

0,8

1

1,2

T.Cruzi cell

lysate

P015 pep de

BSA

H26R peptide M2 peptide

OD (450nm)

Figure 1: Specificity of anti-T.cruzi Immunoglobulin, clone 8E81F4, determined

by ELISA.Antibody diluted to 75 μg/ml in PBS containing 0.05% tween-20 and 1% BSA

was tested on T.cruzi cell lysates, P015 peptide (C-terminal peptide from the

ribosomal P0 protein of T. cruzi),

H26R protein (B1 andreneric receptor; negative control) and M2 peptide (muscarinic

receptor; negative control).

Figure 2: Specificity of anti-T.cruzi Immunoglobulin, clone 8E81F4,

determined by immunofluorescence on T.cruzi trypomastigotes. Cells were

fixed, permeabilized and incubated with MQ7.101 (1,5 μg/ml) and anti-Tc13

(1/500; recognizing a T.cruzi membrane protein). Binding of the antibodies was

detected using anti-mouse IgG Alexa 488 (1/1000) and anti-rabbit IgG Cy3 (1/200).

Acknowledgement

K.A. Gomez and L.M. Tasso

Laboratory of Molecular Biology of Chagas Disease (LaBMECh),

Instituto

de Investigaciones en Ingeniería Genética y Biología Molecular

(INGEBICONICET), Buenos Aires, Argentina.