MQR2.4101 anti-NARS

品名

单克隆人抗天冬酰胺基tRNA

合成酶免疫球蛋白

货号。

MQR 2.4101号

批号。

19048年

数量

100微克

版本：2019年9月11日

预期用途

本产品仅供研究使用。不用于诊断或

治疗程序。

该产品经测试可用于酶联免疫吸附试验

（ELISA）和免疫沉淀（IP）。

提供试剂

抗体以PBS形式提供。

同型

人IgG1-κ

免疫原

天冬酰胺基tRNA合成酶。域：458的V2-P458。

特异性

特异性已在ELISA（图1）和IP-MS中测试。

纯度

纯化蛋白A。

免责声明

该抗体仅供研发使用。不用于诊断或

治疗程序。

注意事项

一。对于专业用户。

2。与任何来自生物源的产品一样

应使用处理程序。

三。该产品可用于不同的技术和

结合不同的样品类型和材料，

因此，每个实验室应验证

应用测试系统。

抗体的制备

使用提供的抗体。

储存/稳定性

储存于-20°C。首次使用后，储存于4°C。避免重复冻融循环。

应用指南

酶联免疫吸附试验：1:1000-1:5000

IP:2微克/毫升

其他用途：由于用途不同，最佳工作稀释度

应在适当的分析中测定产品的含量。

除非实际测试系统的稳定性已经确定，否则

建议在使用前立即稀释产品。

关联

细胞质天冬酰胺tRNA合成酶，一种参与

基本的翻译机制发生在所有的单元中，但也与

抗合成酶综合征的特征是

抗tRNA合成酶的自身抗体。过度进化，蛋白质

已经获得了一个额外的领域，UNE-N，它被认为携带

出非规范函数。

图1：用酶联免疫吸附测定抗NARS的特异性（MQR2.4101）。抗体储备0.48 mg/ml）

在含有0.05%吐温-20和1%牛血清白蛋白的PBS中稀释后，在人天冬酰胺基tRNA上进行试验

合成酶（蓝色）和非靶蛋白（红色）

Product Name

Monoclonal Human anti-asparaginyl-tRNA

synthetase Immunoglobulin

CAT No.

MQR 2.4101

LOT No.

19048

Quantity

100 μg

Edition: September 11, 2019

Intended use

This product is for research use only. NOT for use in diagnostic or

therapeutic procedures.

This product is tested for use in enzyme-linked immunosorbent assay

(ELISA) and immunoprecipitation (IP).

Reagent provided

The antibody is supplied in PBS.

Isotype

Human IgG1κ

Immunogen

Asparaginyl-tRNA synthetase. Domain: V2-P458 of 458.

Specificity

Specificity has been tested in ELISA (figure 1) and IP-MS.

Purity

Protein A purified.

Disclaimer

The antibody is for R&D use only. NOT for use in diagnostic or

therapeutic procedures.

Precautions

1. For professional users.

2. As with any product derived from biological sources, proper

handling procedures should be used.

3. The product may be used in different techniques and in

combination with different sample types and materials,

therefore each individual laboratory should validate the

applied test system.

Preparation of the antibody

Use antibody as supplied.

Storage/Stability

Store at -20°C. After first time use, store at 4°C. Avoid repeated freezethaw cycles.

Application guidelines

ELISA: 1:1000 – 1:5000

IP: 2 μg/ml

Other applications: since applications vary, optimum working dilution

of the product should be determined in the appropriate assay.

Unless the stability in the actual test system has been established, it is

recommended to dilute the product immediately before use.

Relevance

Cytoplasmic asparaginyl-tRNA synthetase, a protein involved in the

basic translation machinery taking place in all cells, but also linked to

anti.synthetase syndrome where the hallmark is the presence of

autoantibodies against a tRNA synthetase. Over evolution, the protein

has achieved an additional domain, UNE-N, which is believed to carry

out non-canonical functions.

Figure 1: Specificity of anti-NARS (MQR2.4101), determined by ELISA. Antibody stock 0.48 mg/ml)

diluted in PBS containing 0.05% tween-20 and 1% BSA was tested on human Asparaginyl-tRNA

synthetase (in blue) and non-target protein (in red)