爱尔兰Megazyme 抗性淀粉检测试剂盒 Resistant Starch Assay Kit K-RSTAR

Colourimetric method for the determination of Resistant Starch 
in cereal products and feeds

Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose + 
maltose

(3) Dissolution of resistant starch pellet in KOH and neutralisation

(α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H2O → D-glucose

(glucose oxidase)
(5) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 120 min (plus overnight incubation)
Detection limit: 2-100% of sample weight
Application examples: 
Plant materials, starch samples and other materials
Method recognition: 
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)

Advantages

• Very cost effective
  

• All reagents stable for > 2 years after preparation
  

• Only enzymatic kit available
  

• Measures enzyme resistant starch
  

• Simple format
  

• Mega-Calc? software tool is available from our website for hassle-free raw data processing
  

• Standard included