BestBac 2.0, v-cath/chiA Deleted Baculovirus Cotransfection Kit

• 91-200
• Molecular Products DNA and Vectors
• The BestBac 2.0, v-cath/chiA Deleted Baculovirus Cotransfection Kit Transfection kit contains everything you need to perform co-transfections with your transfer vector. Included in the kit are:
  
  BestBac 2.0 v-cath/chiA deleted DNA  2.5 ug
  pVL1393-lacZ Control Vector  5 ug     
  Transfection Medium 20 ml
  Expres2TR Transfection Reagent 0.75 ul
  
  The BestBac viral backbone is available in a form that is wild type at the cathepsin and chitinase locus and as a deletion mutant. The v-cath/chiA deletion removes a potentially detrimental protease and may reduce competition for resources for protein synthesis during late gene expression. The v-cath/chiA deletion can improve the integrity of your expressed protein resulting in increased functional protein yield.
  
  Expression Systems’ BestBac DNA provides a means for producing recombinant baculoviruses with efficiencies close to 100%. The principle of this technique lies in the production of baculovirus DNA which contains a deletion in an essential gene. Only co-transfection of insect cells with the viral DNA and a complementing transfer vector can reconstitute a viable virus.
  
  BestBac DNA is derived from the E2 clone of AcMNPV which has been alternately passaged in-vitro and in-vivo to ensure no loss of viral integrity.  The polyhedrin locus of BestBac has been modified to remove only the polyhedrin promoter and coding sequence therefore all polyhedrin gene locus-based baculovirus transfer vectors can be used to complement the BestBac deletion. Transfer vectors include pVL1392, pVL1393 and their derivatives.
• 1.    Molecular Products DNA and Vectors   DNA及载体分子产品