 | Contents:BBB Kit ? (MBT-24H) Thawing - and Incubation - Medium * Millicell? Hanging Cell Culture Insert 4
(for Blank experiments)
| |
| | 货号 | 品牌 | 格式 | 规格
| 价格 | | PCC-MBT-24H | BBB Kit ?
(RBT-24H) | 12 inserts | Set | 25100元 |
|
| * Millicell? is the Registered Trade Mark of Millipore Corporation. |
|
We deliver the complete set of BBB Kit ? in frozen packaged with dry ice. BBB Kit ? can be frozen as a whole and stored at -80℃. We guarantee to use BBB Kit ? (MBT-24H) within 1 month.
4 days prior to your experiment, you just thaw (de-freeze) your BBB Kit ? (MBT-24H) stored at -80℃ in your deep-freezer.
|
| | | | | Amounts of solutions (to be added)
| | Thawing-sol | medium (1) | medium (2) * | | Blood-side | 200 μL | 300 μL | 300 μL | | Brain-side | 1000 μL | 1200 μL | 1200 μL |
* Recipe: medium 10% PDS / DMEM F-12
| Dulbecco's Modified Eagle's Medium/ Nutrient Mixture F-12 Ham (DMEM F-12) |
| | Fetal Bovine Plasma Derived Serum (PDS) | 10% (v/v) | | Heparin | 100 μg/mL | | basic fibroblast growth factor (bFGF) | 1.5 ng/mL | | Insulin-Transferrin-sodium Selenite (ITS) | insulin 5 μg/mL,
transferrin 5 μg/mL,
sodium selenite 5 ng/mL | | Hydrocortisone | 500 nM | | Gentamicin | 50 μg/mL |
|
|
|
| Expression of Tight Junctions (TJs) Proteins in BBB Kit? （MBT-24H） |
| Drug Permeability with BBB Kit? （MBT-24H） |
| Trans Endothelial Electrical Resistance (TEER) of BBB Kit ? (MBT-24H) |
| | TEER in BBB Kit? (MBT-24H) reaches more than 150 Ω x cm2, and maintains a plateau up to 6 days. (Medium does not contain cAMP and its analogs.) TEER is measured at Day 3 after changing medium. BBB Kit? (MBT-24H) can be used from Day 4 to Day 6 after thawing. |  | 【Equipment】
 |
| Insert Membrane Infomation |
| Insert of BBB Kit? (MBT-24H) (Polyethylene terephthalate membrance filter)| Millicell? Hanging Cell Culture Insert (#PISP 12R 48) |  |
| | Technical data of the polyethylene terephthalate (PT )membrane Millicell? Insert (#PISP 12R 48) | | Diameter: | 6.5 mm | | Growth area: | 0.33 cm2 | | Membrane thickness: | 9 μm | | Pore size: | 3.0 μm | | Pore density: | 2 x 106 pores / cm2 | | Tissue culture treated: | yes | | Optical property: | translucent | | Cell visibility: | poor | | Volume inside insert: | 0.2 mL - 0.3 mL | | Volume in plate: | 0.9 mL - 1.2 mL |
|
| Millicell? is the Registered Trade Mark of Millipore Corporation. |
| Contents:BBB Kit ? (MBT-24F) Thawing - and Incubation - medium
| |
| | 货号 | 品名 | 格式 | 规格 | 价格 | PCC-MBT-24F
(On-Demand Product) | BBB Kit ?
(MBT-24F) | 24 inserts | Set | 31000元 |
|
|
