美国Halo色谱柱

Halo多孔壳层色谱柱由国际著名色谱材料专家柯克兰博士（J.J.kirkland.）领衔开发，代表了新一代色谱填料的诞生。创新的熔融核（Fused-CordTM）色谱填料技术，Halo多孔壳层色谱柱采用2.7µm多孔壳层填料，该技术是在直径为1.7µm的实心硅胶球上烧结一层0.5µm的全多孔硅胶层，具有与1.8µm填料相似的分离效率，却只有其1/2的柱压和明显的抗污染性能，与1.8μm粒径柱相比，有更长的使用寿命。柱效可达3μm粒径柱的2倍,将成为新一代高速色谱分析的最佳选择。

Halo色谱柱带来的惊喜：

在普通的液相色谱仪器上得到UPLC的分离速度和效果

大大提高复杂产物的分离度

用50mm的Halo柱可替代150mm的传统5μm粒径色谱柱，还可加快流速1-2倍，提高分离速度6-8倍；节省溶剂60%

明显优于1.7-1.8μm填料的抗污染性和寿命

Halo® column packings are not made the typical way. Instead, the particles packed into HALO® columns are manufactured using Fused-Core® particle technology that was specially developed to deliver hyper-fast chromatographic separations while avoiding the reliability issues so often associated with fast HPLC.

The shorter diffusion path of HALO® particles reduces axial dispersion of solutes and minimizes peak broadening. A Halo particle has only a 0.5 μm diffusion path compared to the approximately1.5 μm diffusion path of a 3 μm totally porous particle. Because of the shorter diffusion path, the performance advantages of HALO® become even more apparent when separating larger solute molecules and operating at faster mobile phase flow rates.

Their ability to generate hyper-fast separations comes not only from their small particle size (2.7 μm) but also from the unique Fused-Core® particle technology that creates a 0.5 μm porous shell fused to a solid core particle. As you increase mobile phase flow rate to speed-up a separation, the slow mass transfer of solute molecules inside the particles can limit resolving power. Fused-Core® particle technology addresses this limitation by providing an incredibly small path (0.5 μm) for diffusion of solutes into and out of the particles, thereby reducing the time solute molecules spend inside the particles and minimizing a major barrier to fast chromatographic separations.

HALO® columns deliver over 50% more separating power (plates) than columns of the same length packed with 3.5 μm particles and more than twice the separating power of columns packed with 5 μm particles.

Note: N/Meter values were calculated at the optimum mobile phase linear velocity for each of these stationary phases. Naphthalene was used as the test probe and the mobile phase conditions used were 60% acetonitrile and 40% water at 24 °C.

Halo® columns deliver over 50% more separating power (theoretical plates) than a column of the same length packed with 3.5 μm particles and more than twice the plates of a column packed with 5 μm particles. And, because of Fused-Core® particle technology, HALO® columns maintain their resolving power at high flow rates. This means that shorter columns and higher flow rates can be used to achieve remarkably fast high resolution separations.

Halo® particles give you all of that separation power without the back pressure seen in sub 2micron columns. In fact, the Halo® particle operate well within the range of typical 400 bar HPLC systems, not requiring you to purchase the expensive ultra-high pressure instruments and develop new laboratory protocols. Halo® columns can turn almost any HPLC system into a high speed workhorse.

Most HPLC systems have operating pressure limits of 6,000 psi (400 bar), or less. As the column packing particle size decreases, the column back pressure increases rapidly. To use columns packed with sub-2 μm size particles at their optimum flow rate, pressure that exceeds 6,000 psi is often encountered. This necessitates purchasing very expensive “ultra-pressure” equipment to achieve optimum performance. HALO® columns, even though they do generate slightly higher back pressure than columns packed with 3.5 μm particles, can be used with most existing HPLC equipment.