ROCHE 罗氏 核酸分离纯化产品-快速体验高质量核酸纯化

应用：

TriPure Isolation Reagent can prepare total RNA, genomic DNA, and protein from a single biological sample. After purification:

• The DNA-free total RNA is suitable for northern blotting, in vitro translation, RNase protection assay, cDNA synthesis, or RT-PCR.
• The RNA-free DNA is suitable for PCR, restriction digests, Southern blotting, or cloning.
• The denatured protein is suitable for SDS-PAGE and western blotting.

原理：

During a one-step sample homogenization/lysis procedure, the TriPure Isolation Reagent disrupts cells and denatures endogenous nucleases. Chloroform is added to the sample and the mixture is centrifuged. This separates the sample into three phases: a colorless aqueous (upper) phase, a white interphase and a red organic (lower) phase. The upper phase is placed in a separate tube and RNA is recovered from it by isopropanol precipitation. DNA and protein are sequentially isolated from the remaining white interphase and red organic phase by alcohol precipitation steps.

相关产品：

• Transcriptor Reverse Transcriptase;
  A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.
• Protector RNase Inhibitor;
  Special Quality for Molecular Biology.
• First Strand cDNA Synthesis Kit for RT-PCR (AMV);
  Kit for first-strand cDNA synthesis as the first step of two-step RT-PCR.
• Transcriptor First Strand cDNA Synthesis Kit;
  For robust two-step RT-PCR of RNA up to 14 kb.