Catalog Number

251069

Unit

2 ml

Price

$275.00

Isotype

Applications

Species reactivity

Format

Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.

Description

The CD25 (HI25a) antibody recognizes a 55 kDa type-I transmembrane glycoprotein known as the low-affinity receptor for IL-2 (IL-2Rα). CD25 associates with CD122 (IL-2Rβ, P75) and the common γ Chain-CD132 (IL-2Rγ, P64) to form the high-affinity IL-2R complex. CD25 antigen is expressed on activated T and B lymphocytes, activated macrophages, and adult T-cell leukemia (ATL) cells, but is absent from resting T and B cells. CD25 antigen density is altered by treatment with PHA, ConA and CD3 antibody.

Product Citations

Tadamitsu K. et al., eds. 1997. Leucocyte Typing VI: White Cell Differentiation Antigens. P49-52, 113-114  Garland Publishing, Inc., New York.

Recommended Products

250957 CD25 (HI25a) Antibody
251013 CD25 (HI25a) Antibody, FITC
 

Alternate Names

Interleukin-2 receptor alpha chain; IL-2 receptor alpha subunit; IL2-RA; IL2-RA; p55; TAC antigen; CD25 antigen; IL2RA

Accession No.

P01589
NP_000409
 

Clonality

HI25a

Application Notes

FACS with whole blood: 100 μl blood + 20 μl antibody; FACS with cells: 10E6 cells + 20 μl antibody. 

FACS: 1:200-1:1,000  

Storage

Store at 4°C. Product is guaranteed 6 months from the date of shipment.