HA(A/California/04/2009(H1N1))

Gene Name Synonym: HA

Protein Construction:A DNA sequence encoding the extracellular domain of Influenza A virus (A/California/04/2009(H1N1)) (ACP41105.1) hemagglutinin（Met 1-Gln 529）(Native, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag.

Source: Influenza A Virus

Expression Host: Human Cells
QC Testing Purity:> 97%, as determined by SDS-PAGE

Endotoxin:< 1.0 EU per 1μg of the protein as determined by the LAL method.

Stability:Samples are stable for up to twelve months from date of receipt -70℃

Predicted N terminal: Asp 18

Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/California/04/2009(H1N1)) comprises 523 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.

Formulation: Supplied as a 0.2μm filtered solution of PBS, pH7.4

SDS-PAGE:
Usage GuideStorage:Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Reconstitution:Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Protein DescriptionHemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. H1N1 is a subtype of influenza virus A and the most common cause of influenza in humans.
References1: Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
2: Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
3: Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.
4: Bonnafous, P. et al., 2000, J. Biol. Chem. 275 : 6160-6166.
 

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