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现在位置：首页>实验室原料试剂耗材>克隆与表达>转染试剂>OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)
OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)
OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)
  • OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)

OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)

产品报价：询价

更新时间：2011/11/9 11:33:40

产地：北京

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型号：欢迎咨询！

厂商性质： 生产型,贸易型,

公司名称： 嘉美诺斯生物科技（北京）有限公司

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在人类基因组测序计划的完成之后，大规模的人类蛋白功能研究和阐明便成为了当今生物医学和制药领域迅速发展的限速步骤。OmicsLink™ ORF 表达克隆系列产品为研究人员提供了70多套各20,000多个高质量的人类基因全长ORF cDNA克隆。 OmicsLink™ ORF 表达克隆可直接用于蛋白表达，生产和纯化，定位及其他多种蛋白功能研究。OmicsLink™ ORF表达克隆在基因组学，蛋白质组学和生物医学以及药物研究和开发领域之间起着重要的纽带和连结作用。

不同的OmicsLink™ ORF Expression Clones，可立即用于在细菌，酵母，昆虫和人类

装载ORF表达克隆的OmicsLink™表达载体系列

 
Human and Mouse Full Length ORF Wheat Germ Cell Free Expression Clones  (8 types)
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pReceiver-WG02 T7 cell free N/A N-His Factor Xa
pReceiver-WG03 T7 cell free N/A N-HisSUMO CoolCutter(TM)
pReceiver-WG04 T7 cell free N/A N-AviSUMO CoolCutter(TM)
pReceiver-WG05 T7 cell free N/A N-HisAviSUMO CoolCutter(TM)
pReceiver-WG09 T7 cell free N/A HisGST TEV
pReceiver-WG16 T7 cell free N/A N/A N/A
pReceiver-WG31 T7 cell free N/A N-HisSUMOAvi CoolCutter(TM)
pReceiver-WG33 T7 cell free N/A N-TrxHisSUMO CoolCutter(TM)
Human and Mouse Full Length ORFEXPRESS™ Gateway® Shuttle Clone(Gateway® pDEST compatible) 
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pDONR™ Vector ORFEXPRESS™ Shuttle Clone
(Gateway® Entry Clone)		 N/A N/A N/A N/A
Human and Mouse Expression Ready Bacterial Full Length ORF cDNA Clones  (14 types)
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pReceiver-B01 T7 E. Coli N/A N-His N/A
pReceiver-B31 T7 E. Coli N/A C-His N/A
pReceiver-B02 T7 E. Coli N/A N/A N/A
pReceiver-B03 T7 E. Coli N/A N-GST Tev
pReceiver-B04 T7 E. Coli N/A N-GST EK
pReceiver-B05 Tac E. Coli N/A N-GST Tev
pReceiver-B06 Tac E. Coli N/A N-GST EK
pReceiver-B07 Tac E. Coli N/A N-MBP Tev
pReceiver-B08 Tac E. Coli N/A N-MBP EK
pReceiver-B09 T7 E. Coli N/A N-Avi N/A
pReceiver-B10 Tac E. Coli N/A N-Flag N/A
pReceiver-B11 Tac E. Coli N/A N-His N/A
pReceiver-B12 Tac E. Coli N/A HisSUMO Sumo protease
pReceiver-B13 T7 E. Coli N/A HisSUMO SUMO Protease
Human and Mouse Expression Ready Mammalian Full Length ORF cDNA Clones  (31 types)
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pReceiver-M01 CMV Mammalian Neomycin N-His N/A
pReceiver-M02 CMV Mammalian Neomycin N/A N/A
pReceiver-M29 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-M03 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-M15 CMV Mammalian Neomycin N-eYFP N/A
pReceiver-M16 CMV Mammalian Neomycin C-eYFP N/A
pReceiver-M32 CMV Mammalian Neomycin N-eCFP N/A
pReceiver-M33 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-M04 CMV Mammalian Neomycin N-GST EK
pReceiver-M05 CMV Mammalian Neomycin N-Avi N/A
pReceiver-M48 CMV Mammalian Neomycin N-Avi+ IRES-Biotin ligase N/A
pReceiver-M17 CMV Mammalian Neomycin C-Avi N/A
pReceiver-M06 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-M07 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-M08 CMV Mammalian Neomycin C-3xHA-His N/A
pReceiver-M45 CMV Mammalian Neomycin C-3xHA+ IRES-eGFP N/A
pReceiver-M09 CMV Mammalian Neomycin C-Myc N/A
pReceiver-M10 CMV Mammalian Neomycin C-Myc-His N/A
pReceiver-M47 CMV Mammalian Neomycin C-Myc+ IRES-eGFP N/A
pReceiver-M11 CMV Mammalian Neomycin N-Flag N/A
pReceiver-M12 CMV Mammalian Neomycin N-3XFlag N/A
pReceiver-M13 CMV Mammalian Neomycin C-Flag N/A
pReceiver-M46 CMV Mammalian Neomycin C-Flag+ IRES-eGFP N/A
pReceiver-M14 CMV Mammalian Neomycin C-3XFlag N/A
pReceiver-M24 CMV Mammalian Neomycin N-SNAP N/A
pReceiver-M26 CMV Mammalian Neomycin C-SNAP N/A
pReceiver-M49 CMV Mammalian Neomycin N-HaloTag Tev protease
pReceiver-M50 CMV Mammalian Neomycin C-HaloTag Tev protease
pReceiver-M55 CMV Mammalian Neomycin N-mCherry N/A
pReceiver-M56 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-M60 CMV Mammalian Neomycin IRES-eGFP N/A
Human and Mouse Expression Ready Lentiviral Full Length ORF cDNA Clones  (39 types)
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pReceiver-Lv01 CMV Stem/primary cell No N/A N/A
pReceiver-Lv31 CMV Stem/primary cell No IRES-eGFP N/A
pReceiver-Lv36 CMV Stem/primary cell No + IRES-luciferase N/A
pReceiver-Lv40 CMV Stem/primary cell Neomycin IRES-Neomycin N/A
pReceiver-Lv21 CMV Stem/primary cell Neomycin N/A N/A
pReceiver-Lv02 CMV Stem/primary cell No C-3xHA N/A
pReceiver-Lv32 CMV Stem/primary cell No C-3xHA + IRES-eGFP N/A
pReceiver-Lv52 CMV Stem/primary cell N/A C-3xHA+IRES2-eGFP N/A
pReceiver-Lv06 CMV Stem/primary cell Neomycin C-3xHA N/A
pReceiver-Lv23 CMV Stem/primary cell Neomycin N-Flag N/A
pReceiver-Lv03 CMV Stem/primary cell No C-Flag N/A
pReceiver-Lv33 CMV Stem/primary cell No C-Flag + IRES-eGFP N/A
pReceiver-Lv53 CMV Stem/primary cell N/A C-Flag+IRES2-eGFP N/A
pReceiver-Lv07 CMV Stem/primary cell Neomycin C-Flag N/A
pReceiver-Lv19 CMV Stem/primary cell Neomycin N-eGFP N/A
pReceiver-Lv04 CMV Stem/primary cell No C-eGFP N/A
pReceiver-Lv08 CMV Stem/primary cell Neomycin C-eGFP N/A
pReceiver-Lv20 CMV Stem/primary cell Neomycin N-eYFP N/A
pReceiver-Lv05 CMV Stem/primary cell No C-eYFP N/A
pReceiver-Lv09 CMV Stem/primary cell Neomycin C-eYFP N/A
pReceiver-Lv34 CMV Stem/primary cell Neomycin N-eCFP N/A
pReceiver-Lv61 CMV Stem/primary cell No C-eCFP N/A
pReceiver-Lv62 CMV Stem/primary cell Neomycin C-eCFP N/A
pReceiver-Lv35 CMV Stem/primary cell No N-Avi + IRES-Biotin ligase N/A
pReceiver-Lv26 CMV Stem/primary cell Neomycin N-Avi N/A
pReceiver-Lv10 CMV Stem/primary cell Neomycin C-Avi N/A
pReceiver-Lv25 CMV Stem/primary cell Neomycin N-Myc N/A
pReceiver-Lv17 CMV Stem/primary cell Neomycin C-Myc N/A
pReceiver-Lv18 CMV Stem/primary cell Neomycin C-Myc-His N/A
pReceiver-Lv43 CMV Stem/primary cell No C-Myc+ IRES-eGFP N/A
pReceiver-Lv70 CMV Stem/primary cell N/A C-Myc+IRES2-eGFP N/A
pReceiver-Lv44 CMV Stem/primary cell No C-Myc+ IRES-eYFP N/A
pReceiver-Lv47 CMV Stem/primary cell Neomycin C-Myc+IRES-Neomycin N/A
pReceiver-Lv64 CMV Stem/primary cell Neomycin N-HaloTag Tev protease
pReceiver-Lv65 CMV Stem/primary cell Neomycin C-HaloTag Tev protease
pReceiver-Lv69 CMV Stem/primary cell N/A C-Myc+IRES-mCherry N/A
pReceiver-Lv71 CMV Stem/primary cell Puromycin N-mCherry N/A
pReceiver-Lv72 CMV Stem/primary cell Neomycin C-mCherry N/A
pReceiver-Lv41 EF1a Stem/primary cell Neomycin N/A N/A
Human and Mouse Expression Ready Yeast Full Length ORF cDNA Clones  (3 types)
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pReceiver-Y01 GAL1 S. cerevisiae N/A C-His N/A
pReceiver-YAD pADH Yeast N/A GAL4AD N/A
pReceiver-YBD pADH Yeast N/A GAL4DB N/A
Human and Mouse Expression Ready Insect Full Length ORF cDNA Clones  (1 type)
Vector Promoter Host Cell Selecting
Marker		 Tag Protease
Site
pReceiver-I01 AcMNPV polyhedrin Insect cell N/A N-His Tev

可用于体内或体外表达

通过简单的“点击－选择－订购”，你便可以立刻用OmicsLink™ORF表达克隆表达你所需要的蛋白并对其进行各类的功能研究。OmicsLink™ORF表达克隆系列产品是构建在70多种带有不同启动子和标签融合蛋白表达载体里。载体中的优化的翻译信号和多种特异标签蛋白使得这些表达克隆特别地适合于蛋白表达，纯化，定位以及其他各类的功能研究。本公司还提供OmicsMate™ 客户特定产品制作和技术咨询服务，构建客户需要但现在不在我们提供的产品目录中的特制克隆。

OmicsLink™ ORF 表达克隆在系统生物学和整合生物学的应用

  •  在E. coli中表达重组蛋白
  •  用反向转染技术在哺乳动物细胞中表达蛋白和细胞定位
  •  在体外无细胞翻译系统中高通量蛋白生产
  •  表达有特异标记的蛋白，用于分离，纯化和固相化及下游功能研究和分析
  •  生产用于组织或器官基因表达谱分析的原位杂交的探针
  •  生产用于基因沉默功能分析的esiRNA模板

OmicsLink™ ORF表达克隆和全长cDNA克隆的区别

Clone Characteristics
OmicsLink™ ORF clones
Whole Transcript cDNA Clones
Gene transcript ORF
(Start to stop only)		 Whole transcripts including 5' and 3' UTRs
Expression ready Yes Some are, most will need further subcloning procedures to remove 5' and 3' UTRs
Mammalian cell transfection
  - Transicent Transfection
  - Stable Transfection		 Yes
Yes		 Only a small number
No
Unique protein tags
  - Protein-Molecule interactions
  - Concentration of lowly expressed proteins
  - Affinity purification of expressed proteins		 Yes
Yes
Yes

Yes

 No
No
No

No
shRNAi validation and rescue Yes No
miRNA and miRNA target validation and rescue Yes No
Produce proteins via in vitro cell-free translation systems Yes No
production Template for esiRNA Yes No
Probe generations for in situ hybridization Yes No

 

质量保证

OmicsLink™ORF表达克隆用经过严格验证的人类全长cDNA克隆和高质量的人体组织cDNA文库作PCR模板构建的。这些高质量OmicsLink™ORF表达克隆在投递前都经过严格的多种QC/QA过程的检测。

* 本产品仅供研究、不用于临床诊断

嘉美诺斯生物科技（北京）有限公司

| 第14年

手机：15313026008

联系人：王小姐

电话：010-57288182

传真：010-80780672

QQ：2512139129

（联系我时，请说明是在来宝网上看到的，谢谢！）

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