北京乐博生物科技有限公司是美国ProSci授权一级代理商，供应其全线产品涵盖了细胞凋亡、信号传导、肿瘤、免疫、细胞生物、神经生物和传染病等领域。产品应用于ELISA、WB、IHC、FACS等，为您的实验提供多样的检测手段，产品应用文献发表在PNAS、Cell research、Blood……使用广泛。

抗体信息如下：

Primary Antibodies

Caspase-8 Antibody

Background

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex (4). The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation (reviewed in 5). FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis (6).

Additional Names

Caspase-8 (CT), FLICE, MACH ,Mch5

	Description Left: Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml. Below: Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/ml.
	Other Product Images

Source

Caspase-8 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

Caspase-8 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Virus Casp-8 / FLICE / MACH / Mch5 (C-Terminus) Peptide (Cat. No. 3473P)

Application

Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 μg/ml. Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3473P - Casp-8 Peptide

Storage

Caspase-8 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1205 - Jurkat Cell Lysate

Species Reactivity

H, M, R

Protein GI Number

15718704

Protein Accession Number

NP_001219

Short Description

(CT) an initiator caspase

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Huntington's Disease Antibodies
• Neurobiology Antibodies
• Autophagy Antibodies
• Cancer Antibodies

Related Products

• Apaf-1 Monoclonal Antibody
  (Catalog No. PM-2015).
• Apaf-1 Monoclonal Antibody
  (Catalog No. PM-2016).
• Caspase-8 Antibody
  (Catalog No. 3475).
• Caspase-8 Peptide
  (Catalog No. 3473P).
• FLIP Antibody
  (Catalog No. 1159).
• Jurkat Cell Slide
  (Catalog No. 17-005).
• Jurkat Lysate
  (Catalog No. 1205).

References

1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2. Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3. Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4. Muzio M, Chinnaiyan AM, Kischkel FC, et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex. Cell 1996; 85:817-27.

更多现货抗体欢迎咨询乐博生物：010-59564854