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抗体信息如下：

Primary Antibodies

Caspase-1 Antibody

Background

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1? and IL-18 (3,4). Caspase-1 was initially identified as an IL-1β-converting enzyme (5); later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 (6) whose overexpression can induce apoptosis in fibroblasts (7).

Additional Names

Caspase-1 (CT), ICE, IL-1beta converting enzyme

	Description Left: Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml. Below: Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 μg/ml.
	Other Product Images

Source

Caspase-1 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human Caspase-1.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

Caspase-1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human Casp-1 / ICE / IL-1b converting enzyme (C-Terminus) Peptide (Cat. No. 3459P)

Application

Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 μg/ml or Immunohistochemistry. Depending on cell lines or tissues used, non-cleaved or other cleavage products may be observed.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3459P - Casp-1 Peptide

Storage

Caspase-1 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1301 - Human Heart Tissue Lysate

Species Reactivity

H

Protein GI Number

15431328

Protein Accession Number

NP_150634

Short Description

(CT) a caspase in the inflammatory subfamily

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Cancer Antibodies

Related Products

• Caspase-1 Antibody
  (Catalog No. 3463).
• Caspase-1 Peptide
  (Catalog No. 3459P).
• Caspase-5 Antibody
  (Catalog No. 3457).
• Heart Lysate
  (Catalog No. 1301).
• Heart Tissue Slide (Normal)
  (Catalog No. 10-501).

References

1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2. Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3. Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
4. Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.

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