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抗体信息如下：

Primary Antibodies

Bcl-2 Antibody

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis (reviewed in 2 and 3). These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis (4). It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad (5 for review). Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer (4,6).

Additional Names

Bcl-2 (IN), Bcl-2

	Description Left: Western blot analysis of Bcl-2 in A-20 cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 μg/ml. Below: Immunocytochemistry of Bcl-2 in A20 cells with Bcl-2 antibody at 2 μg/ml.
	Other Product Images

Source

Bcl-2 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bcl-2.

Purification

Ion exchange chromatography purified

Clonality / Clone

This is a polyclonal antibody.

Host

Bcl-2 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Murine Bcl-2 (Intermediate Domain) Peptide (Cat. No. 3337P)

Application

Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 2 μg/ml.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3337P - Bcl-2 Peptide

Storage

Bcl-2 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1288 - A20 Cell Lysate

Species Reactivity

H

Protein GI Number

20072668

Protein Accession Number

AAH27258

Short Description

(IN) a member of the bcl-2 family

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Neurobiology Antibodies
• Autophagy Antibodies
• Cancer Antibodies

Related Products

• A-20 Cell Slide
  (Catalog No. 17-208).
• A-20 Lysate
  (Catalog No. 1288).
• BAD Antibody
  (Catalog No. 3343).
• Bak Antibody
  (Catalog No. 3347).
• Bcl-2 Antibody
  (Catalog No. 3335).
• Bcl-2 Peptide
  (Catalog No. 3337P).

References

1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4. Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6

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