最经典的RNA提取试剂——MRC公司TRIZOL

关键词：嘉美生物，实验技术服务，Annexin V，重组蛋白，磷酸化抗体，各种动物ELISA试剂盒，RNA干扰，双链短RNA，TRIZOL，Northern分析，RT-PCR

TRI Reagent—最经典的RNA提取试剂，即TRIZOL

☆有效分离RNA，亦可使用同一系统连续纯化DNA及protein
☆快速简便；1小时内可得高纯度之RNA，且回收率较其它方法高30-150 %
☆本产品为Single Step RNA Isolation技术创始人Dr. Piotr Chomczynski所研发，质量值得信赖
☆纯化之RNA可直接用于Northern分析，点杂交，分子筛选，体外转染，RNA酶保护试验，分子克隆，和PCR等实验
☆免DNase处理，不残留protein或DNA
☆可用于动、植物细胞或组织、细菌、全血、病毒，酵母菌之液态或固态样品
☆可搭配BCP使用，取代传统使用之高毒性氯仿(chloroform)
☆于室温可保存长达2年，配方优于其它类似试剂。

附件：TRIZOL的英文介绍

TRI REAGENT - RNA / DNA / PROTEIN ISOLATION REAGENT Cat. No. TR 118
Store at 4 - 25 C

PRODUCT DESCRIPTION
TRI Reagent? is a complete and ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent is the improved version of the popular single-step method of total RNA isolation (1, 2). This highly reliable technique performs well with small and large quantities of tissues or cultured cells and allows simultaneous processing of a large number of samples. TRI Reagent and the single-step method are subjects of the international patents.

TRI Reagent combines phenol and guanidine thiocyanate in a mono-phase solution to facilitate the immediate and most effective inhibition of RNase activity. A biological sample is homogenized or lysed in TRI Reagent and the homogenate is separated into aqueous and organic phases by bromochloropropane (cat. no. BP 151) or chloroform addition and centrifugation. RNA remains exclusively in the aqueous phase, DNA in the interphase and proteins in the organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol, washed with ethanol and solubilized. DNA and proteins are sequentially precipitated from the interphase and the organic phase with ethanol and isopropanol, washed with ethanol and solubilized.

产品订购及咨询，请联系嘉美生物