BioLife Solutions GMP级CryoStor CS5 Freeze Media细胞DMSO冻存液

产品英文名 BioLife Solutions CryoStor CS5 Freeze Media

产品中文名 BioLife Solutions CryoStor CS5细胞DMSO冻存液

产品介绍  BioLife Solutions CryoStor?是BioLife公司特别优化的细胞冻存液，在极低温度 (-70oC to -196oC)下准备和保存细胞。预混DMSO，为细胞和组织的冷冻、储存和解冻过程提供安全的保护环境。CryoStor?通过调控冻存过程的分子生物学反应，无血清、蛋白或具有高细胞毒性的试剂，就能增强细胞活力和功能。

产品订购信息

CryoStor CS5细胞冻存液特点

用于细胞和组织的高级生物保存液

预混DMSO

无血清、无蛋白

USP级高质量成分、cGMP级生产、FDA master文件

灭菌、内毒素和细胞功能检测

DMSO无血清细胞冻存液适用细胞

多种类型细胞，包括如肝细胞之类的敏感细胞

BioLife Solutions CryoStor CS5 细胞DMSO冻存液cGMP级生产

MATERIALS ARE MANUFACTURED UNDER cGMP

CryoStor? Usage and Cryopreservation Protocol使用与冻存方法

细胞准备

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor? solution.

加CryoStor冻存液

4) ISOLATION: Add cold (2-8°C) CryoStor?

a. Cell concentrations: 0.5-10 x 106cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor? - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

储存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

细胞复苏解冻

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.Passive thaw is not recommended.

9) Dilute cell/CryoStor? mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue?) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

BioLife Solutions CryoStor CS5/CS10 Freeze Media 细胞冻存液

CS5和CS10主要区别是规格包装不同。