Contents:

Monoclonal   antibody 6A8, Universal Allergen Standard, Biotinylated antibody 4C1;   sufficient reagents for 10 plates.

Storage:

Store   at 4°C.

Not   Provided:

50mM   Carbonate/Bicarbonate buffer, pH 9.6
  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
  Bovine Serum Albumin
  Streptavidin-Peroxidase
  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Monoclonal   Antibody:

6A8   (clone 6A8 B10 D12)

Vial:

Red   Cap; 100μl

Immunogen:

Der   f 1

Isotype:

Mouse   IgG1

Specificity:

Binds   to a species specific epitope present on Dermatophagoides farinae allergen,   Der f 1.

Purification:

Produced   in tissue culture and purified by affinity chromatography using Protein A.   Single heavy and light chain bands on SDS-PAGE.

Concentration:

1mg/ml   in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml),   0.22μm filtered, preservative free.

Certificate of Analysis

Monoclonal   Antibody:

4C1   (clone 4C1 B8 3F8)

Vial:

Brown   Cap; 100μl

Immunogen:

Der   f 1

Isotype:

Mouse   IgG1

Specificity:

Binds   to a common epitope present on mite Dermatophagoides Group 1 allergens (Der f   1, Der p 1, Der m 1, Eur m 1).

Purification:

Produced   in ascites and purified by affinity chromatography using Protein A. Single   heavy and light chain bands on SDS-PAGE.

Biotinylation:

Biotinylated   and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50%   glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

Certificate of Analysis

Allergen   Standard:

Universal   Allergen Standard

Vial:

White   Cap; 400μl

Composition:

A   formulation of eight purified natural allergens prepared in 1% BSA/50%   glycerol/PBS, pH 7.4

Concentration/Calibration:

Universal   Allergen Standard

Protein   Measurement

Concentration (ng/ml)

Der   p 1

Amino-acid   analysis

2500

Der f 1

Amino-acid analysis

2500

Der   p 2

Amino-acid   analysis

1000

Fel   d 1

Amino-acid   analysis

1000

Can   f 1

Amino-acid   analysis

2500

Rat   n 1

Amino-acid   analysis

1000

Mus   m 1

Amino-acid   analysis

250

Bla   g 2

Amino-acid   analysis

2500

References:

1.     Chapman, M.D., Sutherland, W.M., and Platts-Mills, T.A.E. (1984).    Recognition of two Dermatophagoides pteronyssinus specific epitopes on   Antigen P1 using  monoclonal antibodies:  Binding to each epitope   can be inhibited be sera from mite allergic patients.  J. Immunol.   133:2488-2495.

2.    Ford, A.W., Rawle, F.C., Lind, P., Spieksma, F.Th.M., Lowenstein, H. and   Platts-Mills, T.A.E. (1985). Standardization of Dermatophagoides   pteronyssinus: Assessment of potency and allergen content in ten coded   extracts. Int. Archs. Allergy Appl. Immunol. 76:58-68.

3.    Heymann, P.W., Chapman, M.D. and Platts-Mills, T.A.E. (1986). Antigen Der f 1   from the dust mite Dermatophagoides farinae: Structural comparison with Der p   1 from D. pteronyssinus and epitope specificity of murine IgG and human IgE   antibodies. J. Immunol. 137:2841-2847.

4.     Chapman, M.D., Heyman, P.W., Wilkins, S.R., Brown, M.J., and Platts-Mills,   T.A.E. (1987). Monoclonal immunoassays for major dust mite (Dermatophagoides)   allergens, Der p 1 and Der f 1, and quantitative analysis of the allergen   content of mite and house dust extracts. J. Allergy Clin. Immunol.   80:184-194.

5.    Platts-Mills, T.A.E. and Chapman, M.D. (1987). Dust mites: Immunology,   allergic disease and environmental control. J. Allergy Clin. Immunol.   80:755-775

6.    Chapman, M.D., Heymann, P.W., and Platts-Mills, T.A.E. (1987). Epitope   mapping of two major inhalant allergens, Der p 1 and Der f 1, from mites of   the genus Dermatophagoides. J. Immunol. 139: 1479-1484.

7.    Chapman, M.D. (1988). Allergen specific monoclonal antibodies: New tools for   the management of allerigic disease. Allergy 43 (5):7-14.

8.    Luczynska, C.M., Arruda, L.K., Platts-Mills, T.A.E., Miller, J.D., Lopez, M.,   and Chapman, M.D. (1989). A two-site monoclonal antibody ELISA for the   quantification of the major Dermatophagoides spp. allergens, Der p 1 and Der   f 1. J. Immunol. Meths. 118:227-235.

9.    Platts-Mills T.A.E., Thomas W.R., Aalberse R.C., Vervloet D. and Chapman M.D.   (1992). Dust mite allergens and asthma: Report of a second international   workshop. J Allergy Clin Immunol 89:1046-1060.

10.    Platts-Mills T.A.E., Vervloet D., Thomas W.R., Aalberse R.C., Chapman M.D.   Indoor Allergens and Asthma: Report of the third international workshop. J   Allergy Clin Immunol, 1997; 100:S1-S24.

(For   complete reference list see EL-DF1 Certificate of Analysis)