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Guanidine hydrochloride BioChemica（盐酸胍）
Guanidine hydrochloride BioChemica（盐酸胍）
价 格：询价
产 地：德国更新时间：2021/9/10 14:45:14
品 牌：AppliChem型 号：A1499
状 态：正常点击量：2347
联 系 人：方丽初
电 话：010-88597838
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等 级： (第 17年)
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Guanidine hydrochloride BioChemica
A1499
| Order No. |
Quantity |
| A1499,0250 |
250 |
g |
| A1499,0500 |
500 |
g |
| A1499,1000 |
1 |
kg |
| A1499,10006 |
6 |
x 1 kg |
产品参数
| Synonym |
GuaHCl, Guanidinium chloride |
| Solubility (20°C) |
2000 g/L (H2O) |
| Melting point |
183 - 188°C |
| Formula |
CH5N3 · HCl |
| M |
95.53 g/mol |
| CAS-No. |
50-01-1 |
| HS-No. |
29252900 |
| EC-No. |
200-002-3 |
| Storage |
RT |
| LGK |
10 - 13 |
| Disposal |
3 |
| R |
22-36/38 |
| S |
22 |
 |
harmful, irritant |
| WGK |
1 |
| Specification |
|
| Assay (titr.) |
min. 99 % |
| pH (5 %; H2O; 25°C) |
4.0 - 6.0 |
| Insoluble matter |
complies |
| Water |
max. 0.5 % |
| Ammonium |
max. 0.05 % |
| Fe |
max. 0.0005 % |
| Pb |
max. 0.0005 % |
| A (1 cm/50 % in water HPLC grade) |
|
| 260 nm |
max. 0.1 |
| 280 nm |
max. 0.05 |
产品介绍
(1) MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227
Isolation of RNA using guanidinium salts.
(2) Cockle, S.A. et al. (1978) J. Biol. Chem. 253, 8019-8026
Resistance of lipophilin, a hydrophobic myelin protein, to denaturation by urea and guanidinium salts.
Guanidine hydrochloride denatures proteins and inhibits nucleases. It is applied at high concentrations for the isolation of non-degraded RNA (e. g. stock solution 7.5 M; ref. 1). β-Mercaptoethanol or DTT increase the denaturing activity of guanidine hydrochloride, since they break intramolecular disulfide bonds. Guanidine thiocyanate acts even stronger than guanidine hydrochloride as a denaturant and is used for ''valuable'' samples/assays. Strongly hydrophobic proteins, like the membrane protein lipophilin will not be denatured by guanidine hydrochloride (2).
