脱氧核糖核酸酶I中英文说明书
- 武汉艾美捷科技有限公司2022年6月24日 3:06 点击:876
脱氧核糖核酸酶I中英文说明书
Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.
Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.
Recombinant DNase I is suitable for such applications as:
• Removing genomic DNA from RNA preparations prior to RT-PCR
• Degradation of DNA templates after transcription reactions
• Removing unwanted DNA from samples prior to Northern blotting
• Removing DNA during biopharma and bioprocessing procedures
Unit Definition: One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25oC when acting upon highly polymerized DNA at pH 5.0, which is the same as other Worthington DNase I products.
Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthington. As measured at Worthington, One Kunitz unit digests 1mg of calf thymus (or pUC19 or l-phage) DNA in 10 minutes at 37oC in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8. Correlation of digestion units with Kunitz units may be different in other buffer systems.
Storage Buffer (DR1S): 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.
DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.
艾美捷Worthington脱氧核糖核酸酶I特异性:
bpDNase I 不是碱基或序列特异性的;但是,它不会随机切割。它显示优先在嘧啶的 5' 侧进行裂解,并且在替代共聚物中尤其明显(Bernardi 等人 1975 和 Lomonossoff 等人 1981)。已经表明,扭曲角的变化被 DNase I 识别(Dickerson 和 Drew 1981)。DNase I 的特异性还取决于存在的二价阳离子。在存在 Ca2+ 和 Mg2+ 的情况下,它会导致单链断裂,而在存在 Mn2+ 的情况下会导致双链断裂(Junowicz 和 Spencer 1973,以及 Campbell 和 Jackson 1980)。
脱氧核糖核酸酶I相关研究:
肌动蛋白
白蛋白,无核酸酶
脱氧核糖核酸酶 II
脱氧核糖核酸及相关产品
组蛋白
溶菌酶
核酸酶,微球菌
核酸酶,S1
磷酸酶,碱性
磷酸二酯酶 I
磷酸二酯酶 II
蛋白酶K
逆转录酶,重组 HIV
核糖核酸酶A
核糖核酸酶 T1
核糖核酸酶,E-Rase™ RNase 混合物
核糖核酸
来源:https://www.amyjet.com/products/WBC-LS006362.shtml
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弹性蛋白酶中英文说明书
Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity. Elastase is assayed using a method adapted from that of Feinstein et al., Biochem. Biophys. Res. Comm., 50, 1020 (1973) and using the more soluble substrate of Bieth et al., Biochem. Med., 11, 350 (1974).
Stability/Storage: Elastase is unstable at pH ≤ 3.5. When stored as a dry powder the enzyme is stable for 6-12 months at 2-8°C. Elastase product codes: ES and ESL have poor solubility at neutral pH and at concentrations greater than 0.25%. It is helpful to make primary solutions in KCl or alkaline buffers before diluting into the reaction mixtures or media, compensating for ionic strength or pH changes. Stable at pH 4.0-10.4.
Technical Notes: 1 SucAla3NA unit is approximately equivalent to 6 elastin digestion units. Aqueous liquid suspensions should be aseptically handled to avoid bacterial contamination. Due to the viscous nature of the aqueous suspension (Code: ES) the vial should be rinsed to recover contents.
Unit Definition: One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.
艾美捷Worthington弹性蛋白酶的应用:
组织解离:因为弹性蛋白在结缔组织的弹性纤维中的浓度最高,所以经常使用弹性蛋白酶解离含有广泛细胞间纤维网络的组织。为此,它通常与其他酶一起使用,例如胶原酶、胰蛋白酶和胰凝乳蛋白酶。
弹性蛋白酶相关研究:
胶原酶 (CLS1-4/CLSPA)
脱氧核糖核酸酶 I (DP/D/DCLS/D2/DPFF/DPRF)
透明质酸酶 (HSE/HSEP)
中性蛋白酶(分散酶,NPRO)
木瓜蛋白酶 (PAP/PAPL/PAP2)
胃蛋白酶 (PM)
蛋白酶 K (PROK)
胰蛋白酶 (TL/TRL/TRL3/TRLS/TRTPCK)
胰蛋白酶抑制剂 (LBI/OI/SI/SIC)
细胞分离优化系统 (CIT)
肝细胞分离系统 (HIS)
新生儿心肌细胞分离系统 (NCIS)
木瓜蛋白酶解离系统 (PDS/PDS2)
来源:https://www.amyjet.com/products/WBC-LS006367.shtml
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木瓜蛋白酶中英文说明书
Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. Stabilizing agents are EDTA, cysteine and dimercaptoethanol.
Stability/Storage: Stable for 6-12 months at 2-8°C. Do not freeze aqueous suspensions.
Technical Notes: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.
Unit Definition: One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.
木瓜蛋白酶功能特性:
木瓜蛋白酶主要用于啤酒抗寒(水解啤酒中的蛋白质,避免冷藏后引起的浑浊)、肉类软化(水解肌肉蛋白和胶原蛋白,使肉类软化)、谷类预煮的准备、水解蛋白质和肉类香料的生产。在啤酒抗寒和肉类软化方面的应用远比其他蛋白酶类广泛。
艾美捷Worthington木瓜蛋白酶特异性:
木瓜蛋白酶具有相当广泛的特异性;它具有内肽酶、酰胺酶和酯酶活性。活性位点由七个亚位点(S 1 -S 4和 S 1' -S 3')组成,每个亚位点可以容纳底物的一个氨基酸残基(P 1 -P 4和 P 1' -P 3')(Schechter和伯杰 1967)。特异性由 S 2亚位点控制,S 2 亚位点是一个容纳底物 P 2侧链的疏水袋。木瓜蛋白酶表现出特定的底物偏好,主要是对该子位点的大量疏水或芳香残基(Kimmel 和 Smith 1954)。在 S 2之外子位点偏好,活性位点内缺乏明确定义的残基选择性。
木瓜蛋白酶相关研究:
胶原酶 (CLS1-4/CLSPA)
脱氧核糖核酸酶 I (DP/D/DCLS/D2/DPFF/DPRF)
透明质酸酶 (HSE/HSEP)
中性蛋白酶 (Dispase, NPRO)
蛋白酶 K (PROK)
胰蛋白酶 (TL/TRL/TRL3/TRLS /TRTPCK)
胰蛋白酶抑制剂 (LBI/OI/SI/SIC)
细胞分离优化系统 (CIT)
肝细胞分离系统 (HIS)
新生儿心肌细胞分离系统 (NCIS)
木瓜蛋白酶解离系统 (PDS/PDS2)
来源:https://www.amyjet.com/products/WBC-LS003128.shtml
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脱氧核糖核酸酶I中英文说明书
Enzymatic Reaction:
Bovine pancreatic deoxyribonuclease is an endonuclease that preferentially splits phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5’-phosphate terminated polynucleotides with a free hydroxyl group at the 3’ position. DNase I is secreted by exocrine glands, and found most abundantly in the pancreas and parotid. It is also present in lower quantities in other tissues (Chen and Liao 2006, and Nadano et al. 1993).
DNase is known to be involved in apoptosis and has been proposed to play a role in the regulation of actin polymerization in cells. In addition to its use in molecular biology, DNase I has been used as a treatment for cystic fibrosis, and systemic lupus erythematosus (Chen and Liao 2006).
Specificity:
bpDNase I is not base nor sequence specific; however, it does not cleave randomly. It shows preference for cleavage at the 5’ side of pyrimidines, and is particularly pronounced in alternative copolymers (Bernardi et al. 1975, and Lomonossoff et al. 1981). It has been shown that variations in the twist angle are recognized by DNase I (Dickerson and Drew 1981). The specificity of DNase I also depends on the divalent cations present. In the presence of Ca2+ and Mg2+, it causes single strand breaks, and in the presence of Mn2+ double strand breaks have been reported (Junowicz and Spencer 1973, and Campbell and Jackson 1980).
Applications:
DNA removal in primary cell isolation: decreases viscosity providing better yields
DNA removal in bioprocessing applications
Removing genomic DNA from RNA preparations prior to RT-PCR
in vitro transcription
Nick translation
DNase footprinting
Actin binding
UV crosslinking of proteins to nucleic acids
Radioactive labeling
艾美捷Worthington脱氧核糖核酸酶I特异性:
bpDNase I 不是碱基或序列特异性的;但是,它不会随机切割。它显示优先在嘧啶的 5' 侧进行裂解,并且在替代共聚物中尤其明显(Bernardi 等人 1975 和 Lomonossoff 等人 1981)。已经表明,扭曲角的变化被 DNase I 识别(Dickerson 和 Drew 1981)。DNase I 的特异性还取决于存在的二价阳离子。在存在 Ca2+ 和 Mg2+ 的情况下,它会导致单链断裂,而在存在 Mn2+ 的情况下会导致双链断裂(Junowicz 和 Spencer 1973,以及 Campbell 和 Jackson 1980)。
艾美捷Worthington脱氧核糖核酸酶I分子特征:
与人类、小鼠和大鼠类似,牛胰腺 DNase I 基因由 9 个外显子组成,只有最后 8 个外显子编码该蛋白质(De María 和 Arruti 2003)。新生蛋白通过由外显子二编码的 22 个氨基酸信号序列引导至分泌途径细胞器。主要活性位点残基 H166 由外显子 6 编码(De María 和 Arruti 2003 和 Kraehenbuhl 等人 1977)。尽管牛和其他哺乳动物的外显子长度几乎相同,但内含子的长度差异很大。TATA 盒序列位于外显子 I 上游 35 bp(De María 和 Arruti 2003)。已经提出影响编码序列的多重剪接事件可能是下调 DNase I 表达的机制(Liu 等人 1997)。
脱氧核糖核酸酶I相关研究:
肌动蛋白
白蛋白,无核酸酶
脱氧核糖核酸酶 II
脱氧核糖核酸及相关产品
组蛋白
溶菌酶
核酸酶,微球菌
核酸酶,S1
磷酸酶,碱性
磷酸二酯酶 I
磷酸二酯酶 II
蛋白酶K
逆转录酶,重组 HIV
核糖核酸酶A
核糖核酸酶 T1
核糖核酸酶,E-Rase™ RNase 混合物
核糖核酸
来源:https://www.amyjet.com/products/WBC-LS006362.shtml
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胰蛋白酶中英文说明书
Enzymatic Reaction:
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains (Brown and Wold 1973). The enzyme in excreted by the pancreas and takes part in the digestion of food proteins and other biological processes. Trypsin is a medium-sized globular protein and is produced as an inactive proenzyme, trypsinogen (Chen et al. 2009).
Specificity:
Trypsin cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. If a proline residue is on the carboxyl side of the cleavage site, the cleavage will not occur. If an acidic residue is on either side of the cleavage site, the rate of hydrolysis has been shown to be slower.
Applications:
Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase
Cell harvesting by “trypsinization”
Mitochondria isolation
in vitro studies of proteins
Removing monolayers of cells from plastic and glass
Various hemagglutination procedures
Sample preparation for flow cytometric DNA analysis
Tryptic mapping
Fingerprinting and sequencing work
Environmental monitoring
Reduction of cell density in tissue culture
Subculturing cells
Cleavage fusion proteins
Generating glycopeptides from purified glycoproteins
艾美捷Worthington胰蛋白酶特异性:
胰蛋白酶切割赖氨酸和精氨酸氨基酸残基 C 端侧的肽。如果脯氨酸残基位于切割位点的羧基侧,则不会发生切割。如果酸性残基位于裂解位点的任一侧,则水解速率已显示较慢。
胰蛋白酶相关研究:
细胞分离优化系统
胰凝乳蛋白酶
胶原酶
脱氧核糖核酸酶 I
内切蛋白酶Lys-C
肝细胞分离系统
透明质酸酶
新生儿心肌细胞分离系统
中性蛋白酶(分散酶)
木瓜蛋白酶解离系统
蛋白酶K
胰蛋白酶抑制剂
来源:https://www.amyjet.com/products/WBC-LS02124.shtml
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Abnova丨5-甲基胞嘧啶多克隆抗体中英文说明
Product Description:Sheep polyclonal antibody raised against 5-Methyl Cytosine.
Immunogen:5-methyl cytosine.
Host:Sheep
Form:Liquid
Recommend Usage:Immunohistochemistry (10 ug/mL)
Immunoprecipitation (25-100 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Storage Buffer:In 150 mM PBS, pH 7.4 (no preservatives)
Storage Instruction:Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
艾美捷Abnova 5-甲基胞嘧啶多克隆抗体 :针对 5-甲基胞嘧啶产生的绵羊多克隆抗体。
免疫原:5-甲基胞嘧啶。
宿主:羊
形式:液体
推荐用法:免疫组织化学 (10 ug/mL)
免疫沉淀 (25-100 ug/mL)
Western Blot (1:1000)
存储缓冲区:在 150 mM PBS 中,pH 7.4(无防腐剂)
储存说明:储存在-20°C。
分装以避免反复冻融。
艾美捷Abnova 5-甲基胞嘧啶多克隆抗体应用:
蛋白质印迹
免疫组织化学
免疫沉淀
免疫荧光:
用 PBS 中的 2% BSA/1% Tween-20 阻断大鼠神经元细胞的阳性染色,并在 4°C 下用 5 ug/mL 稀释度的 5-甲基胞嘧啶多克隆抗体(货号 PAB24932)孵育过夜。
Abnova丨5-甲基胞嘧啶多克隆抗体相关研究:
MAB1738
3-DG-咪唑啉酮单克隆抗体,克隆 JNH 27
MAB2405
4-氨基联苯单克隆抗体,克隆 4C11
MAB6115
4-HNE单克隆抗体
MAB3635
5-溴脱氧尿苷 (BrdU) 单克隆抗体,克隆 MoBu-1
MAB15116
5-羟甲基胞嘧啶 (5-hmC) 单克隆抗体,克隆 RM236
MAB15115
5-甲基胞嘧啶 (5-mC) 单克隆抗体,克隆 RM231
Abnova通过基因组学手段生产出的几万种重组蛋白和抗体,极大的扩展了生物试剂应用的视野和多样性。致力于推动生命科学研究发展与药物研发等领域。艾美捷科技是Abnova的中国代理商,为科研工作者提供优质的产品与服务。
来源:https://www.amyjet.com/brand/abnova-china-distributor.shtml
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Abnova丨A4GNT多克隆抗体中英文说明
Product Description:Rabbit polyclonal antibody raised against recombinant A4GNT.
Immunogen:Recombinant protein corresponding to amino acids of human A4GNT.
Sequence:NFVEHYNSAIWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPSFNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELG
Host:Rabbit
Reactivity:Human
Form:Liquid
Purification:Antigen affinity purification
Isotype:IgG
Recommend Usage:Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.
Storage Buffer:In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction:Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note:This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
艾美捷Abnova A4GNT多克隆抗体 :针对重组 A4GNT 产生的兔多克隆抗体。
免疫原:对应于人 A4GNT 氨基酸的重组蛋白。
宿主:兔子
反应性:人类
形式:液体
纯化:抗原亲和纯化
同种型:IgG
推荐用法:免疫组织化学 (1:50-1:200)
存储缓冲区:在 PBS 中,pH 7.2(40% 甘油,0.02% 叠氮化钠)
储存说明:储存于 4°C。在-20°C 下长期储存。
分装以避免反复冻融。
艾美捷Abnova A4GNT多克隆抗体应用:
免疫组织化学(福尔马林/PFA 固定石蜡包埋切片)
用 A4GNT 多克隆抗体(货号 PAB20405)对人胃进行免疫组织化学染色显示,在 1:50-1:200 稀释度下,腺细胞中的细胞质呈强阳性。
艾美捷Abnova A4GNT多克隆抗体相关研究:
5-PAB21985
6-AADACL1多克隆抗体:针对重组 AADACL1 产生的兔多克隆抗体。
7-PAB28608
8-AADAC多克隆抗体:针对重组 AADAC 产生的兔多克隆抗体。
9-H00008086-M02
10-AAAS 单克隆抗体 (M02),克隆 5A1:针对部分重组 AAAS 产生的小鼠单克隆抗体。
11-PAB28545
12-A2M多克隆抗体:针对重组 A2M 产生的兔多克隆抗体。
13-MAB10476
14-A1BG 单克隆抗体,克隆 4B5:针对部分重组 A1BG 产生的小鼠单克隆抗体。
Abnova通过基因组学手段生产出的几万种重组蛋白和抗体,极大的扩展了生物试剂应用的视野和多样性。致力于推动生命科学研究发展与药物研发等领域。艾美捷科技是Abnova的中国代理商,为科研工作者提供优质的产品与服务。
来源:https://www.amyjet.com/brand/abnova-china-distributor.shtml
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Abnova丨CSV 磁珠中英文说明
Product Description:CSV (cell-surface vimentin) monoclonal antibody conjugated magnetic beads for cell separation and purification.
Particle Core:Iron oxide (Fe3O4)
Particle Coating:Hydrophilic layer of glycidyl ether
Surface Modification:CSV monoclonal antibody
Particle Size:1 um in diameter
Storage Buffer:In PBS, pH 7.4 (0.1% BSA, 0.1% Tween 20, and 0.09% sodium azide)
Storage Instruction:Stored at 2-8°C.
艾美捷Abnova CSV 磁珠 :用于细胞分离和纯化的 CSV(细胞表面波形蛋白)单克隆抗体偶联磁珠。
粒子核心:氧化铁(Fe 3 O 4)
粒子涂层:缩水甘油醚亲水层
表面改性:CSV 单克隆抗体
粒径:直径 1 微米
存储缓冲区:在 PBS 中,pH 7.4(0.1% BSA、0.1% Tween 20 和 0.09% die氮化钠)
储存说明:储存于 2-8°C。
艾美捷Abnova CSV 磁珠应用:
纯化
艾美捷Abnova CSV 磁珠相关研究:
U0381
PSMA磁珠:PSMA 单克隆抗体偶联磁珠用于细胞分离和纯化。
U0014
96深孔板(聚丙烯):96 深孔板用于 Precipitor™ 32 仪器的应用。
KA3267
MaxBead 羧基标记试剂盒:MaxBead Carboxyl Labeling Kit 包含将抗体、蛋白质或核酸等配体偶联到磁珠上所需的所有试剂。
U0084
MaxBead 蛋白 A:MaxBead Protein A 是一种磁珠,设计用于通过蛋白 A-IgG-抗原相互作用捕获 IgG 或抗原。
Abnova通过基因组学手段生产出的几万种重组蛋白和抗体,极大的扩展了生物试剂应用的视野和多样性。致力于推动生命科学研究发展与药物研发等领域。艾美捷科技是Abnova的中国代理商,为科研工作者提供优质的产品与服务。
来源:https://www.amyjet.com/brand/abnova-china-distributor.shtml
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Abnova丨BSG 单克隆抗体中英文说明
Product Description:Mouse monoclonal antibody raised against full length recombinant human BSG.
Immunogen:Recombinant protein corresponding to full length human BSG.
Host:Mouse
Theoretical MW (kDa):35, 40
Reactivity:Human
Form:Liquid
Purification:Protein A/G purification
Isotype:IgG1
Recommend Usage:Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1:100-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
Storage Buffer:In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction:Store at 4°C.
Note:This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
艾美捷Abnova BSG 单克隆抗体 :针对全长重组人 BSG 产生的小鼠单克隆抗体。
免疫原:对应于全长人 BSG 的重组蛋白。
宿主:鼠
理论分子量(kDa):35, 40
反应性:人类
形式:液体
纯化:蛋白 A/G 纯化
同种型:IgG1
推荐用法:流式细胞术(0.1 mL 中0.5-1 ug/10 6 个
细胞)免疫荧光(1:100-1:200)
免疫组织化学(福尔马林/PFA 固定石蜡包埋切片)(0.5-1 ug/mL)
存储缓冲区:在 10 mM PBS(0.05% BSA、0.05% 叠氮化钠)中。
储存说明:储存于 4°C。
艾美捷Abnova BSG 单克隆抗体应用:
免疫组织化学(福尔马林/PFA 固定石蜡包埋切片)
艾美捷Abnova BSG 单克隆抗体相关研究:
MAB13442
BSG 单克隆抗体,克隆 BSG/963:针对全长重组人 BSG 产生的小鼠单克隆抗体。
H00000836-D01
CASP3 MaxPab 兔多克隆抗体 (D01):针对全长人 CASP3 蛋白产生的兔多克隆抗体。
MAB20624
CASP3 单克隆抗体,克隆 AOB-3:针对人 CASP3 合成肽的兔单克隆抗体。
MAB19525
CASP3 单克隆抗体,克隆 DGI-3:针对人 CASP3 合成肽的兔单克隆抗体。
MAB20625
CASP3 单克隆抗体,克隆 GAG-3:针对人 CASP3 合成肽的兔单克隆抗体。
MAB19526
CASP3 单克隆抗体,克隆 HE-3:针对人 CASP3 合成肽的兔单克隆抗体。
Abnova通过基因组学手段生产出的几万种重组蛋白和抗体,极大的扩展了生物试剂应用的视野和多样性。致力于推动生命科学研究发展与药物研发等领域。艾美捷科技是Abnova的中国代理商,为科研工作者提供优质的产品与服务。
来源:https://www.amyjet.com/brand/abnova-china-distributor.shtml
联系邮箱:kefu@labbase.net
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