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艾美捷Fluorochorome荧光金——超广泛、超有效

武汉艾美捷科技有限公司2023年2月8日 3:40 点击:411

艾美捷Fluorochorome荧光——超广泛、超有效

 

Founded in Denver, Co, USA in 1985, fluorochrome has been responsible for the research and development of fluorescent dyes, and its patented product fluoro gold (Fluorogold) has been widely used worldwide since its introduction in 1985, with a large number of references. Fluorogold, which gives a blond light upon UV excitation, is characterized as being very sensitive and labels not only the cytosol but also well displays dendritic branching, but nuclei and nucleoli do not stain; Slow decomposition within the soma, with no significant change in labeling intensity even surviving for 2 months post injection; Compared to UV resistant irradiation, fading is slower; Can be subjected to many histological staining procedures and thus can be combined with HRP, immunohistochemistry, and other methods. In parallel, fluorochrome has developed highly sensitive fluoro gold antibodies, as well as fluoro Ruby (red fluorescent gold) and others, expanding its use.

 

Fluoro Gold & fluoro Ruby - gold standard for retrograde / anterograde tracing of neurons:

Fluorogold (fluorescein), trade name fluoro gold, is the most widely used and effective neuronal retrograde tracer worldwide;

Fluoro gold (Fluorogold) antibodies are by far the most effective antibodies against Fluorogold and have made impressive gains since fluorochrome was first introduced in 1998, becoming the industry standard;

Fluoro Ruby (red fluorescent gold) is a rapid, sensitive, reliable, and technically simple fluorescent neuronal anterograde tracer, and the use of fluoro ruby is essentially identical to fluoro gold and is considered the leading neuronal anterograde tracer.

 

艾美捷Fluorochorome研究工具在生命科学实验有着广泛的应用,Fluorochorome致力于荧光染料的研究与开发,其Fluoro Gold(荧光金)自1985年推出以来被广泛应用,并有大量的参考文献。除此之外,Fluorochrome还拥有高灵敏度的Fluoro-Gold抗体、以及 Fluoro-Ruby(红色荧光金)等等。

 

Fluorochorome Fluoro-Gold & Fluoro-Ruby ——神经元逆行/顺行示踪标准:

FluoroGold( 荧光金),品名Fluoro-Gold,是使用广泛、有效的 神经元逆行示踪剂

Fluoro-Gold( 荧光金)抗体是有效的针对于荧光金的抗体,自Fluorochrome于1998年推出以来,它取得了非常好的成绩

Fluoro-Ruby( 红色荧光金)是一种快速、灵敏、可靠且技术简单的荧光神经元顺行示踪剂,Fluoro-Ruby的使用于Fluoro-Gold基本相同,被认为是了不起的神经元顺行示踪剂

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Fluorochorome
热门研究: 

荧光金 Fluoro-Gold 20mg

荧光金 Fluoro-Gold 50mg

荧光金抗体 Antibody to Fluoro-Gold 0.1ml×1

红色荧光金 Fluoro-Ruby 30mg

Ki067 Antibody-W 200ul

GFAP Antibody-W 200ul

BrdU Antibody-W 200ul

 

来源:https://www.amyjet.com/news/Fluorochrome-n.shtml

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血红素加氧酶-1(HO-1) 单克隆抗体,助力科学研究

 

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Alternative Name: HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1

Clone: HO-1-1

Host: Mouse

Isotype: IgG1κ

Immunogen: Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.

UniProt ID: P09601

GenBank ID: X14782

Gene/Protein Identifier: 3162 (Entrez GeneID), 141250 (OMIM)

Source: Purified from ascites.

Species reactivity: Human, Mouse, Rat

Bovine, Dog

Applications: Flow Cytometry, ICC, IHC (PS), WB

Recommended Dilutions/Conditions: Flow Cytometry (10µg/ml)

Western Blot (1:1,000 ECL)

Suggested dilutions/conditions may not be available for all applications.

Optimal conditions must be determined individually for each application.

Application Notes: Detects a band of ~32kDa by Western blot.

Purity Detail: Protein G affinity purified.

Formulation: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Shipping: Shipped on Blue Ice

Long Term Storage: -20°C

 

血红素加氧酶-1 (HO-1) 也称为 Hsp32,是血红素加氧酶的诱导亚型,可催化血红素的 NADPH、氧气和细胞色素 P450 还原酶依赖性氧化为一氧化碳、亚铁和胆绿素,并迅速还原为胆红素。HO反应的这些产物具有重要的生理作用:一氧化碳是一种有效的血管扩张剂,并且被认为是cGMP和血管张力的生理调节剂;胆绿素及其产物胆红素是有效的抗氧化剂;“游离”铁通过影响铁调节蛋白(IRP)-1的构象及其与mRNA的1'或5'-UTR中的铁调节元件(IRE)的结合,增加氧化应激并调节许多mRNA(例如DCT-3,铁蛋白和转移受体)的表达。

 

艾美捷血红素加氧酶-1(HO-1) 单克隆抗体ADI-OSA-110-D)相关参数:

替代名称: HMO

免疫原: 对应于人HO-1N末端附近序列的合成肽。

基因/蛋白质标识符: 3162 (Entrez GeneID), 141250 (OMIM)

源: 从腹水中纯化。

物种反应性: 人类, 老鼠, 鼠牛,

应用: 流式细胞术, ICC, IHC (PS), WB

推荐稀释度/条件: 流式细胞术(10μg/ml)蛋白质印迹(1:1,000 ECL)

建议的稀释度/条件可能不适用于所有应用。

应用说明: 通过蛋白质印迹检测~32kDa的条带。

纯度细节: 蛋白G亲和纯化。

配方: 液。在含有50%甘油和0.09%叠丨氮丨化钠的PBS中。

航运: 在蓝冰上运输

长期储存: -20°C

 

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图:HO-1 Hsp32)、单克隆抗体 (HO-1-1) 的蛋白质印迹分析(产品编号ADI-OSA-110):车道 1MW 标记;泳道 2HO-1(大鼠)、(重组)(产品编号ADI-SPP-730;车道 3HO-1(人)、(重组)(产品编号ADI-SPP-732;车道 4HO-2(人)、(重组)(产品编号ADI-SPP-550;泳道5MDBK细胞裂解物;车道6:狗肝微粒体;车道7:小鼠肝脏微粒体。

 

血红素加氧酶-1(HO-1) 单克隆抗体ADI-OSA-110-D)部分文献参考:

A preliminary attempt to establish multiple stress response profiles of human skin fibroblasts exposed to mild or severe stress during ageing in vitro: S.I.S. Ratten, et al.; Mech. Ageing Dev. 170, 92 (2018);

Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats: N.E. Buglak, et al.; Redox Biol. 19, 166 (2018);

Dysregulated heme oxygenase-1low M2-like macrophages augment lupus nephritis via Bach1 induced by type I interferons: D. Kishimoto, et al.; Arthritis Res. Ther. 20, 64 (2018);

Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model: Q. Han, et al.; Lipids Health Dis. 17, 255 (2018);

Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells: Y.L. Lai, et al.; Redox Biol. 15, 51 (2018);

Lutzomyia longipalpis Saliva Induces Heme Oxygenase-1 Expression at Bite Sites: N.F. Luz, et al.; Front. Immunol. 9, 2779 (2018);

Neuroprotective effect of an Nrf2-ARE activator identified from a chemical library on dopaminergic neurons: Y. Izumi, et al.; Eur. J. Pharmacol. 818, 470 (2018),

 

来源:https://www.amyjet.com/products/ADI-OSA-110-D.shtml

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多功能艾美捷钙网蛋白(CRT)单克隆抗体

 

The multifunctional, multi -compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins, as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the solub le paralog of the ER membrane protein Calnexin (Cnx). Crt's three domains include a 180 residue N-terminal domain, a proline-rich Pdomainresidues 189 -288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P -domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N -glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.

 

多功能、多区室蛋白钙网蛋白(Crt)可作为新的或错误折叠的蛋白质的可溶性分子伴侣,以及Ca2+结合蛋白。Crt 表达在 ER 管腔中最为丰富,也发生在其他膜结合细胞器、细胞表面和细胞外。Crt 也称为 CRP-55、钙调节蛋白和 HACBP(高亲和力钙结合蛋白),含有 ER 检索序列 KDEL,是 ER 膜蛋白钙连接蛋白 (Cnx) 的可溶物。

 

CRT 似乎也可作为系统性红斑狼疮、类风湿性关节炎、乳糜泻、完全性先天性心脏传导阻滞和氟烷肝炎的自身抗原发挥作用。Crt 的多种附加功能包括粘附、血液功能以及心脏和神经元发育基因表达。

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艾美捷钙网蛋白(CRT)单克隆抗体ADI-SPA-601PE-D)基本参数:

克隆: FMC 75

宿主:

同种型: 免疫球蛋白G1

免疫原: 重组钙网蛋白。

源: 从腹水中净化

物种反应性: 人类

猴子

应用: 流式细胞术

推荐稀释度/条件: 流式细胞术 1:100)

建议的稀释度/条件可能不适用于所有应用。

应用说明: 预测分子量为~63kDa。

纯度细节: 蛋白G亲和纯化。

配方: 液。在PBS中,pH 7.2,含有0.09%叠丨氮丨化钠。

处理: 避免冻融循环。避光。

航运: 蓝冰不结冰

长期储存: +4°C

 

钙网蛋白(CRT)单克隆抗体部分文献引用:

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol. 208, 1371 (2022);

IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer 20, 647 (2020); Full Text

Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun. 9, 3194 (2018);

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol. 208, 1371 (2022);

IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer 20, 647 (2020); Full Text

Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun. 9, 3194 (2018);

 

钙网蛋白(CRT)单克隆抗体相关研究:

钙网蛋白单克隆抗体 FMC 75)ADI-SPA-601-F

钙网蛋白单克隆抗体 FMC 75)(DyLight™ 488 偶联物) ADI-SPA-601-488-F

小鼠 IgG1 同种型对照,单克隆抗体 (MOPC-21)(PE 偶联物) ADI-SAB-600PE-F

 

来源:https://www.amyjet.com/products/ADI-SPA-601PE-D.shtml

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兽医学动物模型研究:Kingfisher Biotech艾美捷解决方案

 

艾美捷Kingfisher Biotech针对各种兽医学动物模型,所研究的物种多达60种研究动物。目前主要提供的动物物种,为牛,犬,山羊,鲶鱼,鸡,食蟹猴,海豚,马,猫,豚鼠,仓鼠,人,小鼠,绵羊,兔,大鼠,猪,火鸡,斑马鱼等。

 

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Kingfisher Biotech重点研究:

重组蛋白:干扰素、白介素、巨噬细胞移动抑制因子等

抗体:抗CCL2、抗IL、抗CXCL9等多抗,尤其是CD系列流式抗体齐全

ELISA开发试剂盒:牛CCL2、牛IL-13、犬和马IL-1 beta、鸡IL-16等ELISA试剂盒(抗体对+抗原标准品)

 

Kingfisher Biotech部分研究列表:

Anole IGF-1(酵母衍生重组蛋白)-25微克(Kingfisher,CAT#RP1675A-025)

Anole IGF-1(酵母衍生重组蛋白)-5微克(Kingfisher,CAT#RP1675A-005)

Anole IGF-2(酵母衍生重组蛋白)-100微克(Kingfisher,CAT#RP1676A-100)

Anole IGF-2(酵母衍生重组蛋白)-25微克(Kingfisher,CAT#RP1676A-025)

Anole IGF-2(酵母衍生重组蛋白)-5微克(Kingfisher,CAT#RP1676A-005)

Anole IGF-2(酵母衍生重组蛋白)-500微克(Kingfisher,CAT#RP1676A-500)

食蟹猴白血病抑制因子(LIF)(酵母衍生的重组蛋白)-100微克(Kingfisher,目录号RP1074Y-100)

食蟹猴白血病抑制因子(LIF)(酵母衍生的重组蛋白)-25微克(Kingfisher,目录号RP1074Y-025)

食蟹猴白血病抑制因子(LIF)(酵母衍生的重组蛋白)-5微克(Kingfisher,目录号RP1074Y-005)

食蟹猴白血病抑制因子(LIF)(酵母衍生的重组蛋白)-500微克(Kingfisher,目录号RP1074Y-500)

IGF-1(酵母衍生的重组蛋白)-100微克(Kingfisher,目录号RP1330-100)

IGF-1(酵母衍生的重组蛋白)-25微克(Kingfisher,目录号RP1330-025)

IGF-1(酵母衍生的重组蛋白)-500微克(Kingfisher,目录号RP1330-500)

IGF-1(酵母衍生重组蛋白)-5微克(Kingfisher,CAT#RP1330-005)

食蟹猴CCL4(MIP-1 beta)多克隆抗体(Kingfisher,CAT#KP1690Y-100)

食蟹猴CCL4(MIP-1 beta)多克隆抗体-生物素化(Kingfisher,CAT#KPB1691Y-050)

食蟹猴CCL4(MIP-1β)自己做ELISA,≤10板(Kingfisher,CAT#DIY1692Y-003)

蜜蜂防御素-1(酵母衍生重组蛋白)-25微克(Kingfisher,CAT#RP1644AP-025)

蜜蜂防御素-1(酵母衍生重组蛋白)-5微克(Kingfisher,CAT#RP1644AP-005)

IL-17A(酵母衍生重组蛋白)-100微克(Kingfisher,CAT#RP0056B-100)

IL-17A(酵母衍生重组蛋白)-25微克(Kingfisher,CAT#RP0056B-025)

IL-17A(酵母衍生重组蛋白)-5微克(Kingfisher,CAT#RP0056B-005)

IL-17A(酵母衍生重组蛋白)-500微克(Kingfisher,CAT#RP0056B-500)

IL-4(酵母衍生的重组蛋白)-100微克(Kingfisher,CAT#RP0001B-100)

IL-4(酵母衍生重组蛋白)-25微克(Kingfisher,CAT#RP0001B-025)

IL-4(酵母衍生的重组蛋白)-5微克(Kingfisher,CAT#RP0001B-005)

IL-4(酵母衍生重组蛋白)-500微克(Kingfisher,CAT#RP0001B-500)

PDGF-BB(酵母衍生的重组蛋白)-5微图(Kingfisher,目录号RP1319B-005)

/山羊/绵羊IL-5(酵母来源的重组蛋白)-25微克(Kingfisher,CAT#RP0139B-025)

 

来源:https://www.amyjet.com/news/Kingfisher-Biotech.shtml

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可靠标记就找它——艾美捷Fluorochrome Ki67抗体

 

Ki67 is an intrinsic marker of ongoing cell proliferation and is present during all active phases of the cell cycle (G1-M). This antibody is polyclonal and was raised in rabbit to the human peptide sequence – TPKEKAQALEDLAGFKELFQT – that was coupled to thyroglobulin by glutaraldehyde before being injected into the rabbit. The antibody has been used for immunocytochemistry in rat and human brain tissue. The vial contains about 200ul at a dilution of 1/100. The antibody can be further diluted to at least 1/20,000 in BSA diluent (50mM KPBS, 0.4% Triton, 1% BSA, 1% NGS). The antibody should be stored at -20C. Also included is filter paper absorbed with the specific peptide for blocking. 500ul of the diluted antibody can be added to the tube to achieve a 10 uM block. Incubate for at least 1hr at room temperature (RT) before applying to tissue. The antibody blocks specifically with this peptide and does not block with other peptides absorbed on filter paper

 

ki67是一种增殖细胞的相关抗原,其功能与有丝分裂密切相关,在细胞增殖中是不可缺少的。所以在临床上ki67主要用于标记增殖周期中的细胞,该标记(+)率高这主要是由肿瘤生长越快,组织分化能力越差,而对ki67也越敏感。一般来说这样的肿瘤,预后都是较差的,通常很难完全治愈。所以说晚期的肿瘤由于其分裂较快而细胞的分化较不完善,从而造成ki67的敏感性增高。

 

Ki-67抗原为细胞核内分裂增殖相关蛋白, 在细胞周期S、G1、G2、M期均有表达, G0期缺如, 常作为肿瘤细胞增殖活性的可靠标记。

 

艾美捷Fluorochrome Ki67抗体FC40001)是多克隆抗体,在兔体内产生人肽序列TPKEKAQALEDLAGFKELFQT,该肽序列在注射入兔体内之前通过戊二醛与甲状腺球蛋白偶联。该抗体已用于大鼠和人脑组织中的免疫细胞化学。该小瓶含有约200ul,稀释度为1/100。可以在BSA稀释剂(50mM KPBS,0.4%Triton,1%BSA,1%NGS)中将抗体进一步稀释至至少1/20000。抗体应储存在-20℃。还包括用特定肽吸收的滤纸用于封闭。可以将500ul稀释的抗体加入管中以获得10μm的块。在应用于组织之前,在室温(RT)下孵育至少1小时。该抗体特异性地阻断该肽,并且不阻断滤纸上吸收的其他肽

 

Fluorochrome Ki67抗体FC40001)基参:

中文名称:Ki67抗体

英文名字:Ki67 Antibody-W

规格:200ul

应用类型:IHC

免疫原:该抗体是多克隆抗体,在兔体内针对人肽序列TPKEKAQALEDLAGFKELFQT产生

来源宿主:兔

反应性:人,大鼠

保存建议:小瓶中含有约200微升1/100稀释液。 抗体可在BSA稀释液(50 mM KPBS、0.4%Triton、1%BSA、1%NGS)中进一步稀释至至少1/20,000。 抗体应在-20 ℃下储存。 还包括吸附有用于封闭的特异性肽的滤纸。 可向管中加入500 μ l稀释的抗体以获得10 μ M阻断。 应用于组织前,在室温(RT)下孵育至少1小时。 抗体特异性地与该肽阻断,而不与吸附在滤纸上的其他肽阻断。

 

艾美捷Fluorochrome Ki67抗体FC40001)特点:

各批次间结果一致且可重复

采用重组技术,可实现快速生产

经过大量验证确认了特异性

不含动物成分

 

Ki67抗体FC40001)参考文献

Perez et al, The Journal of Neuroscience, May 13, 2009:29(19):6379-6387

Garcia-Fuster et al, in preparation: Individual differences in adult hippocampal neurogensis following experimenter administration of cocaine.

 

来源:https://www.amyjet.com/products/FC40001.shtml

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GroPep Bioreagents IGF-I 系列:生物素人IGF-I

 

艾美捷GroPep Bioreagents生物素人胰岛素样生长因子-I(生物素基IGF-I)是通过人IGF-I与磺基-NHS-生物素试剂反应以标记IGF-I分子的伯胺而制成的。产生生物素化形式的混合物,主要物种是二生物素基IGF-I。生物素基IGF-I已用间隔物制备,以最大程度地减少随后亲和素结合过程中的空间位阻。

 

61.png

 

GroPep Bioreagents生物素人IGF-I基本参数

参考文献Grulich-Henn J等(1998)激素研究49,1-7

来源:在大肠杆菌中生产。

分子量:主要种类是二生物素IGF-I(8327道尔顿)。单生物素IGF-I(7988道尔顿)和三生物素IGF-I(8683道尔顿)

也在场

纯度:人IGF-I(受体级)(通过HPLC分析>95%)是

源材料

生物活性:IGFBP-2的Western配体印迹

外观:白色粉末用乙腈/0.1%TFA冷冻干燥并储存

在氮气下轻微真空

储存/稳定性:在2-4°C下至少2年(作为冷冻干燥产品)

重组:处理GroPep IGF-I,IGF-II和IGF类似物

检测:使用生物素-IGF-I或IGF-II进行蛋白质配体印迹的程序。

 

GroPep Bioreagents生物素人IGF-I相关研究:

单生物素人IGF-II(受体级)

二生物素-人IGF-II(受体级)

IGF-I(受体级)

人类IGF-I(媒体级)

IGFBP-2

 

GroPep Bioreagents在分子生物领域、发酵学及重组蛋白和单抗制备方面拥有非常熟练的技术,GroPep专注于细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂等研究领域。

 

来源:https://www.amyjet.com/news/GroPep-new.shtml

————————————————————

GroPep Bioreagents 鲷鱼胰岛素样生长因子-I方案

 

艾美捷GroPep Bioreagents鲷鱼胰岛素样生长因子-I(IGF-I)是一种单链67氨基酸多肽,与人IGF-I具有结构同源性。它与[Gly相同1] 金头鲷 (Sparus aurata) IGF-I 和 [Gly1] 黑鲷 (刺荚蒾) IGF-I 作为甘氨酸取代了这些 IGF 中的丝氨酸。鲷鱼 IGF-I 可用于各种体内和体外应用, 包括那些研究生长激素的作用。

 

61.png

 

GroPep Bioreagents生物素人IGF-I基本参数

参考文献:Duguay SJ等(1996)Journal of Molecular Endocrinology 16123-132

来源:在大肠杆菌中生产。

分子量:7379道尔顿

纯度:>90%(通过HPLC分析)

N端序列:5个残基的分析

生物活性:刺激大鼠L6成肌细胞中的蛋白质合成

ED50<50 ng/ml)

内毒素:<0.1 EU/μg

外观:白色粉末用0.1M乙酸冷冻干燥并储存在

氮气在轻微真空下。

储存/稳定性:在2-4°C下至少2年(作为冷冻干燥产品)。

重组:处理GroPep IGF-I,IGF-II和IGF类似物

检测:IGF肽碘化程序

通过以下方法测定一系列物种中的IGF-I或IGF-II:

放射免疫分析(RIA)

 

GroPep Bioreagents生物素人IGF-I相关研究:

Barramundi IGF-I抗血清(兔)

鲑鱼/鳟鱼IGF-I

Barramundi IGF-I

 

GroPep Bioreagents在分子生物领域、发酵学及重组蛋白和单抗制备方面拥有非常熟练的技术,GroPep专注于细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂等研究领域。

 

来源:https://www.amyjet.com/news/GroPep-new.shtml

————————————————————————

GroPep Bioreagents 艾美捷IGF-I 系列鸡肉 IGF-I

 

艾美捷GroPep Bioreagents鸡胰岛素样生长因子-I(IGF-I)是一种单链70氨基酸多肽,与人IGF-I和鸡IGF-II具有结构同源性。与人IGF-I的序列相比,八种氨基酸是不同的。鸡 IGF-I 已被证明可以刺激许多细胞类型的生长和分化, 包括鸡胚胎成纤维细胞.受体级鸡 IGF-I 是一种高质量的研究试剂,特别适用于细胞和动物生长研究, 用于鸡 IGF-I 的特定测定和其他一般研究目的。

 

61.png

 

GroPep Bioreagents生物素人IGF-I基本参数

参考文献:Upton Z等(1992)Journal of Molecular Endocrinology 9,83-92

McMurtry JP等(1997)家畜内分泌学14,

199-229

来源:在大肠杆菌中生产。

分子量:7738道尔顿

纯度:>95%(通过HPLC分析)

N端序列:5个残基的分析

生物活性:IGF I型受体结合试验(ED50<10ng/ml);

刺激大鼠L6成肌细胞中的蛋白质合成

ED50<30 ng/ml)

内毒素:<0.1 EU/μg

外观:白色粉末用0.1M乙酸冷冻干燥并储存在

氮气在轻微真空下。

储存/稳定性:在2-4°C下至少2年(作为冷冻干燥产品)。

重组:处理GroPep IGF-I,IGF-II和IGF类似物

检测:IGF肽碘化程序

通过以下方法测定一系列物种中的IGF-I或IGF-II:

放射免疫分析(RIA)

使用碘化IGF-I或IGF-I进行蛋白质配体印迹的程序

 

GroPep Bioreagents生物素人IGF-I相关研究:

鸡肉 IGF-II

IGF-I多克隆抗体(兔)

 

GroPep Bioreagents在分子生物领域、发酵学及重组蛋白和单抗制备方面拥有非常熟练的技术,GroPep专注于细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂等研究领域。

 

来源:https://www.amyjet.com/news/GroPep-new.shtml

 

 

 

 

 

(来源: 武汉艾美捷科技有限公司


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