BXC-SIM0010体内SIM人类PD-1抗体（帕博利珠单抗生物类似药）

BXC-SIM0010体内SIM人类PD-1抗体（帕博利珠单抗生物类似药）

Product Details

This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Pembrolizumab making it ideal for research use. This Pembrolizumab biosimilar reacts with human PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In experimental models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being used as cancer treatments. Pembrolizumab binds to PD-1 on activated immune cells to selectively block the interaction of PD-1 with its ligands.

BXC-SIM0010体内SIM人类PD-1抗体（帕博利珠单抗生物类似药）：

这种非治疗性生物仿制药抗体使用与治疗性抗体Pembrolizumab相同的可变区域，使其成为研究用途的理想选择。这种Pembrolizumab生物仿制药与人PD-1（程序性死亡-1）反应，也称为CD279。PD-1是由Pdcd50基因编码的55-1 kDa细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。

BioXCell艾美捷体内SIM人类PD-1抗体（帕博利珠单抗生物类似药）规格：

#  BXC-SIM0010

Isotype Human IgG4

Recommended Isotype Control(s) RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Human PD-1

Reported Applications Blocking of PD-1/PD-L signaling

Functional assays

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from cell culture supernatant

Purification Protein A

RRID AB_2894731

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 人免疫球蛋白G4

推荐的同种型对照 RecombiMAb人IgG4（S228P）同种型对照，抗母蛋溶菌酶

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 人类PD-1

报告的应用程序 阻断PD-1/PD-L信号传导

功能检测

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <1EU/mg （<0.001EU/μg）

通过LAL凝胶凝血测定法测定

集合体 <5%

由 DLS 确定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从细胞培养上清液中纯化

纯化 蛋白A

里德 AB_2894731

分子量 150 千达

鼠病原体检测 外切粒菌/鼠痘病毒： 阴性 汉坦病毒： 阴性 K 病毒： 阴性乳酸脱氢酶升高病毒： 阴性 淋巴细胞性脉络丛脑膜炎病毒： 阴性小鼠腺病毒： 阴性小鼠巨细胞病毒： 阴性小鼠肝炎病毒： 阴性小鼠分钟病毒： 阴性小鼠诺如病毒： 阴性小鼠细小病毒： 阴性

小鼠轮状病毒：肺支原体阴性：阴性 小鼠肺炎病毒：阴性 多瘤病毒：阴性呼肠孤病毒筛查：阴性 仙台病毒：阴性 泰勒鼠脑脊髓炎：阴性

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-SIM0010-1mg.shtml

——————————————————————————

BioXCell BE0146体内单克隆抗小鼠 PD-1(CD279)抗体

Product Details

This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Pembrolizumab making it ideal for research use. This Pembrolizumab biosimilar reacts with human PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In experimental models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being used as cancer treatments. Pembrolizumab binds to PD-1 on activated immune cells to selectively block the interaction of PD-1 with its ligands.

RMP1-14单克隆抗体与小鼠PD-1（程序性死亡-1）反应，也称为CD279。PD-1是由Pdcd50基因编码的55-1 kDa细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。此外，Pdcd1 mRNA在前B细胞阶段的发育B淋巴细胞中表达。

BioXCell艾美捷体内单克隆抗小鼠 PD-1(CD279)抗体规格：

#  BXC-BE0146

Isotype Human IgG4

Recommended Isotype Control(s) RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Human PD-1

Reported Applications Blocking of PD-1/PD-L signaling

Functional assays

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from cell culture supernatant

Purification Protein A

RRID AB_2894731

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 大鼠IgG2a， κ

推荐的同种型对照 体内MAb大鼠IgG2a同种型对照，抗三硝基苯酚

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 用小鼠PD-1 cDNA转染的叙利亚仓鼠BKH细胞

报告的应用程序 PD-1/PD-L信号传导的体内阻断

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

里德 AB_10949053

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-CP162-1mg.shtml

————————————————————————————

BioXCell BE0101体内单克隆抗小鼠PD-L1(B7-H1)说明书

Product Details

The 10F.9G2 monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and PD-1. The 10F.9G2 antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80).

BioXCell 艾美捷BE0101体内单克隆抗小鼠PD-L1(B7-H1)抗体:

10F.9G2单克隆抗体与小鼠PD-L1（程序性死亡配体1）反应，也称为B7-H1或CD274。PD-L1是一种40 kDa的I型跨膜蛋白，属于Ig超家族的B7家族。PD-L1在T淋巴细胞，B淋巴细胞，NK细胞，树突状细胞以及IFNγ刺激的单核细胞，上皮细胞和内皮细胞上表达。PD-L1与其受体PD-1结合，PD-4存在于CD8和CD1胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上。

BioXCell艾美捷体内单克隆抗小鼠PD-L1(B7-H1)抗体规格：

#  BXC-BE0101

Isotype Rat IgG2b, κ

Recommended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin

Recommended Dilution Buffer InVivoPure pH 6.5 Dilution Buffer

Immunogen Mouse CD274

Reported Applications in vivo PD-L1 blockade

Immunofluorescence

Immunohistochemistry (frozen)

Flow cytometry

Western blot

Formulation PBS, pH 6.5

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

RRID AB_10949073

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 大鼠IgG2b， κ

推荐的同种型对照 体内MAb大鼠IgG2b同种型对照，抗锁孔帽贝血蓝蛋白

推荐的稀释缓冲液 体内纯 pH 6.5 稀释缓冲液

免疫原 鼠标 CD274

报告的应用程序 体内PD-L1阻断

免疫荧光

免疫组化（冷冻）

流式细胞术

蛋白质印迹

配方 PBS，pH 6.5

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

里德 AB_10949073

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-BE0101-1mg.shtml

——————————————————————————

BioXCell RecombiMAb小鼠PD-1(CD279)抗体(D265A)

Product Details

The RMP1-14-CP151 monoclonal antibody is a chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical to the original RMP1-14 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The RMP1-14-CP151 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. RMP1-14-CP151 reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.

BioXCell RecombiMAb小鼠PD-1(CD279)抗体(D265A)：

RMP1-14-CP151单克隆抗体是原始RMP1-14抗体的嵌合版本。可变结构域序列与原始RMP1-14相同，但恒定区域序列已从大鼠IgG2a切换到小鼠IgG2a。RMP1-14-CP151抗体在Fc片段中还含有D265A突变，使其无法与内源性Fcγ受体结合。RMP1-14-CP151与小鼠PD-1（程序性死亡-1）反应，也称为CD279。PD-1是由Pdcd50基因编码的55-1 kDa细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。

BioXCell艾美捷RecombiMAb小鼠PD-1(CD279)抗体(D265A)规格：

#  BXC-CP151

Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) RecombiMAb mouse IgG2a (D265A) isotype control, anti-hen egg lysozyme

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Mutations D265A

Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

Reported Applications in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from CHO cell supernatant in an animal free facility

Purification Protein G

RRID AB_2927525

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 小鼠IgG2a， κ

推荐的同种型对照 RecombiMAb小鼠IgG2a（D265A）同种型对照，抗母蛋溶菌酶

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

突变 D265A

免疫原 用小鼠PD-1 cDNA转染的叙利亚仓鼠BKH细胞

报告的应用程序 体内阻断PD-1/PD-L信号传导**

报道了原始大鼠IgG2a RMP1-14抗体

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <1EU/mg （<0.001EU/μg）

通过LAL凝胶凝血测定法测定

集合体 <5%

由 DLS 确定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的CHO细胞上清液中纯化

纯化 蛋白 G

里德 AB_2927525

分子量 150 千达

鼠病原体检测 外切粒菌/鼠痘病毒： 阴性 汉坦病毒： 阴性 K 病毒： 阴性乳酸脱氢酶升高病毒： 阴性 淋巴细胞性脉络丛脑膜炎病毒： 阴性小鼠腺病毒： 阴性小鼠巨细胞病毒： 阴性小鼠肝炎病毒： 阴性小鼠分钟病毒： 阴性小鼠诺如病毒： 阴性小鼠细小病毒： 阴性

小鼠轮状病毒：肺支原体阴性：阴性 小鼠肺炎病毒：阴性 多瘤病毒：阴性呼肠孤病毒筛查：阴性 仙台病毒：阴性 泰勒鼠脑脊髓炎：阴性

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-CP151-1mg.shtml

——————————————————————————————

BioXCell RecombiMAb小鼠PD-1(CD279)抗体说明书

Product Details

The RMP1-14-CP162 monoclonal antibody is a chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical to the original RMP1-14 but the constant region sequences have been switched from rat IgG2a to mouse IgG1. The RMP1-14-CP162 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. RMP1-14-CP162 reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.

BioXCell RecombiMAb小鼠PD-1(CD279)抗体：

RMP1-14-CP162单克隆抗体是原始RMP1-14抗体的嵌合版本。可变结构域序列与原始RMP1-14相同，但恒定区域序列已从大鼠IgG2a切换到小鼠IgG1。RMP1-14-CP162抗体不含Fc突变，就像原始大鼠IgG2a抗体不含一样。 RMP1-14-CP162与小鼠PD-1（程序性死亡-1）反应，也称为CD279。PD-1是由Pdcd50基因编码的55-1 kDa细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。

此外，Pdcd1 mRNA在前B细胞阶段的发育B淋巴细胞中表达。PD-1的结构包括ITIM（基于免疫受体酪氨酸的抑制基序），表明PD-1负调节TCR信号。PD-1通过结合其两个配体PD-L1和PD-L2来发出信号，PD-L7和PD-L1都是B1家族的成员。配体结合后，PD-1信号传导抑制T细胞活化，导致增殖减少，细胞因子产生和T细胞死亡。此外，已知PD-1在小鼠外周耐受和预防自身免疫性疾病中起关键作用，因为PD-1基因敲除动物表现出扩张型心肌病，脾肿大和外周耐受性丧失。诱导PD-L8表达在许多肿瘤中很常见，包括鳞状细胞癌、结肠腺癌和乳腺腺癌。

BioXCell艾美捷RecombiMAb小鼠PD-1(CD279)抗体规格：

#  BXC-CP162

Isotype Mouse IgG1, κ

Recommended Isotype Control(s) InVivoPlus mouse IgG1 isotype control, unknown specificity

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

Reported Applications in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from CHO cell supernatant in an animal free facility

Purification Protein A

RRID AB_2927529

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 小鼠IgG1， κ

推荐的同种型对照 体内加小鼠IgG1同种型对照，特异性未知

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 用小鼠PD-1 cDNA转染的叙利亚仓鼠BKH细胞

报告的应用程序 体内阻断PD-1/PD-L信号传导**

报道了原始大鼠IgG2a RMP1-14抗体

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <1EU/mg （<0.001EU/μg）

通过LAL凝胶凝血测定法测定

集合体 <5%

由 DLS 确定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的CHO细胞上清液中纯化

纯化 蛋白A

里德 AB_2927529

分子量 150 千达

鼠病原体检测 外切粒菌/鼠痘病毒： 阴性 汉坦病毒： 阴性 K 病毒： 阴性乳酸脱氢酶升高病毒： 阴性 淋巴细胞性脉络丛脑膜炎病毒： 阴性小鼠腺病毒： 阴性小鼠巨细胞病毒： 阴性小鼠肝炎病毒： 阴性小鼠分钟病毒： 阴性小鼠诺如病毒： 阴性小鼠细小病毒： 阴性

小鼠轮状病毒：肺支原体阴性：阴性 小鼠肺炎病毒：阴性 多瘤病毒：阴性呼肠孤病毒筛查：阴性 仙台病毒：阴性 泰勒鼠脑脊髓炎：阴性

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-CP162-1mg.shtml

————————————————————————————

BioXCell体内MAb小鼠/人磷酸化PD-1(CD279)抗体介绍

Product Details

The 407.6G12 monoclonal antibody reacts with phosphorylated mouse PD-1. Specifically, the antibody only reacts with PD-1 when tyrosine 248 (Y248) is phosphorylated. PD-1 is a 50-55 kDa cell surface receptor that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. PD-1 signals via binding its two ligands, PD-L1 and PD-L2. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Phosphorylation of Y248 of the ITSM motif of PD-1 is essential for the initiation of inhibitory signals. PD-1 blockade leads to reduced phosphor-PD-1. The 6G12 antibody can be used to assess the efficacy of PD-1 pathway blockade.

BioXCell体内MAb小鼠/人磷酸化PD-1(CD279)抗体：

407.6G12单克隆抗体与磷酸化小鼠PD-1反应。具体来说，抗体仅在酪氨酸1（Y248）磷酸化时与PD-248反应。PD-1是一种50-55 kDa的细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。

BioXCell艾美捷体内MAb小鼠/人磷酸化PD-1(CD279)抗体规格：

#  BXC-BE0387

Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Human PD-1 p248Tyr peptide conjugated to KLH

Reported Applications Western Blot

Flow Cytometry

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 µm filtration

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C.

同种型 小鼠IgG2a， κ

推荐的同种型对照 体内MAb小鼠IgG2a同种型对照，特异性未知

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 与KLH偶联的人PD-1 p248Tyr肽

报告的应用程序 蛋白质印迹

流式细胞术

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μm 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冷冻。

来源：https://www.amyjet.com/products/BXC-BE0387-1mg.shtml