抗体/靶向药物内化检测丨艾美捷ZAP抗体内化试剂盒

抗体/靶向药物内化检测丨艾美捷ZAP抗体内化试剂盒

抗体药物偶联物（Antibody-Drug Conjugate，ADC）是通过一个化学链接将具有生物活性的细胞毒性药物连接到单抗上。单抗作为载体将小分子药物靶向投递到靶细胞上，与细胞表面的抗原结合后发生内吞，小分子药物随之进入靶细胞并发挥毒性效应。

艾美捷ZAP抗体内化试剂盒 （仅适用于一抗） 与用户提供的一抗结合，形成二级偶联毒素，再靶向细胞表面的抗原，从而筛选真正起作用的靶向物。

【案例】ZAP抗体内化试剂盒丨gPIG-ZAP [IT-64, KIT-64]

gPIG ZAP使用豚鼠的一级抗体来靶向和消除细胞。该二级缀合物使用二级抗体（亲和纯化的山羊抗豚鼠IgG）“背驮”到豚鼠一级抗体上。gPIG ZAP可用于筛选豚鼠IgG抗体以用于内化和/或其制备有效免疫毒素的适用性。当体外结果证实了所需的特异性时，建议定制抗体与saporin的结合。

gPIG-ZAP是亲和纯化的山羊抗豚鼠IgG和核糖体失活蛋白saporin的化学偶联物。gPIG ZAP使用豚鼠亲和纯化的多克隆抗体来靶向和消除细胞。该二级缀合物用于评估一级抗体内化的潜力。

ZAP抗体内化试剂盒相关研究：

注：gPIG ZAP可单独提供（#IT-64）或作为试剂盒（#kit-64）提供，其中包括gPIG ZAP、Saporin（#PR-01）、山羊IgG SAP（#IT-19）和用于开发细胞毒性测定的试剂。 

来源：https://www.amyjet.com/featured/ADC.shtml

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抗体内化能力方案丨艾美捷ZAP生物素化内化试剂盒研究

单克隆抗体目前被广泛用于抗癌、抗炎和抗病毒治疗。评价一款抗体药物有效性和安全性的一个重要特性是细胞内化的程度、位置和速度。抗体药物偶联物（Antibody-Drug Conjugate，ADC）是通过一个化学链接将具有生物活性的细胞毒性药物连接到单抗上。抗体内化能力是 ADC药物早期筛选的一项重要指标。

ZAP生物素化内化试剂盒 （适用于生物素化一抗及其他靶向剂） 与用户提供的生物素化靶向剂（如一抗）结合，再靶向细胞表面的抗原，从而筛选真正起作用的靶向物。

ZAP生物素化内化试剂盒适用的靶向物类别包括：

○一抗

○多肽

○蛋白配体

○核酸适配体

○细胞因子

○其它任何与靶细胞表面结合的靶向物

ZAP生物素化内化试剂盒艾美捷体内测定，靶向剂案例展示：

靶向剂种类：Streptavidin-ZAP (KIT-27-A)

该试剂盒包括用于主要抗体宿主物种的Streptavidin ZAP（IT-27）和BIgG SAP对照缀合物。

链亲和素ZAP是链亲和素和核糖体失活蛋白saporin的化学偶联物。它将生物素化物质转化为目标毒素。

该试剂盒将生物素化物质转化为靶向毒素，并允许评估试剂与受体结合后内化的能力。它是体内筛选生物素化抗体的完-美工具。

图：KNRK细胞以2500个细胞/孔接种孵育过夜。链霉亲和素ZAP是与生物素化-IB4等摩尔预混浓度。空白Streptavidin SAP，IB4-然后以10ul体积加入SAP和Streptavidin ZAP+生物素化dB4混合物培养板72小时。XTT/PMS混合物以50ul的体积添加到每个孔和板中在450nm下读取，并使用Prism进行数据分析软件（GraphPad，圣地亚哥）。

文献参考：

1. Sheriff ST, Xiao C, Chance WT, Kasckow JW, Balasubramaniam A (2002) Selective lesion of

neuropeptide Y (NPY)-receptor neurons in hypothalamus inhibit food intake and reduces body weight inrats. Soc Neurosci Mtg, Orlando FL, Abstract #384.1.

2. Kohls M (2006) Evaluate Potential Targeting Molecules. Nature Methods.

来源：https://www.amyjet.com/featured/ADC.shtml

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定量测定多物种PGE2——艾美捷PGE2酶联免疫试剂盒方案

Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.

Sensitivity: 13.4 pg/ml (range 39.1 - 2,500 pg/ml)

Assay Time: <3 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include cerebral spinal fluid, dialysate, gingival crevicular fluid, peritoneal exudate fluid and peritoneal exudate cell supernatant, plasma, and tissue.

Wavelength: 405 nm

Species reactivity: Species independent

Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.

Shipping: Shipped on Blue Ice

Contents: GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

PGE2酶联免疫试剂盒：用于炎症和类二十烷研究的高灵敏度PGE2 ELISA试剂盒。

PGE2酶联免疫试剂盒化学性质：

替代名称：前列腺素E2

灵敏度：13.4 pg/ml（范围39.1-2500 pg/ml）

化验时间：<3小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种的培养上清液、血清、唾液、尿液和全血中的PGE2。引用的样本类型包括脑脊髓液、透析液、龈沟液、腹膜渗出液和腹膜渗出细胞上清液、血浆和组织。

波长：405 nm

物种反应性：物种无关

使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。

运输：蓝冰运输

内容：GxM IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

PGE2酶联免疫试剂盒文献参考：

Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment: G. Ruiz-Heiland, et al.; Clin. Oral. Investig. 25, 1933 (2021), Abstract; Full Text

Potential Surrogate Quantitative Immunomodulatory Potency Assay for Monitoring Human Umbilical Cord-derived Mesenchymal Stem Cells Production: S. Teng, et al.; Cell Biol. Int. 45, 1072 (2021), Abstract;

Synergistic effect of Korean red ginseng extract and GABA mixture on the IgE production in mice via Th1/Th2 cell balance: J.S. Lim, et al.; Food Sci. Biotechnol. 30, 1571 (2021), Abstract;

The inhibitory effect of AMP-activated protein kinase (AMPK) on chemokine and prostaglandin production in human endometrial stromal cells: Y. Kawano, et al.; Reprod. Biol. Endocrinol. 19, 188 (2021),

来源：https://www.amyjet.com/products/ADI-900-001.shtml

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广泛引用各类样类型——艾美捷TXB2酶联免疫试剂盒

The TXB2 ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Detect COX I inhibition by measuring the production of downstream TXB2 in a convenient 96 well plate based assay. This kit is highly cited in peer-reviewed publications in a diverse assortment of sample types including mucosa, tissue, serum, plasma, urine, and much more. The TXB2 ELISA kit provides easy-to-use stable liquid color-coded reagents that reduce errors.

Alternative size available: ADI-901-002 (5x96 wells)

High sensitivity measurement, detecting as little as 10.54 pg/ml of TXB2

High throughput format with results in < 3 hours for up to 37 samples in duplicate

Widely cited in peer-reviewed publications in a diverse assortment of sample types including mucosa, tissue, serum, plasma, urine, and much more

Reliable and reproducible results day-after-day and lot-after-lot

艾美捷TXB2酶联免疫试剂盒是一种比色竞争酶免疫测定试剂盒，结果在<3小时内。在405 nm处读取吸光度。通过在方便的96孔板基测定中测量下游TXB2的产生来检测COX I抑制。该试剂盒在各种样本类型的同行评审出版物中被高度引用，包括粘膜、组织、血清、血浆、尿液等。TXB2 ELISA试剂盒提供易于使用的稳定液体颜色编码试剂，减少了错误。

TXB2酶联免疫试剂盒化学性质：

备选名称：血栓素B2

灵敏度：10.54 pg/ml（范围13.7-10000 pg/ml）

化验时间：<3小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种的培养上清液、血浆、血清、唾液和尿液中的TXB2。引用的样本类型包括细胞裂解液、冠状动脉流出物、肝脏灌注液、血小板和全血。

波长：405 nm

物种反应性：物种无关

运输：蓝冰未冻结

长期储存：+4°C

内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

TXB2酶联免疫试剂盒特点：

1.高灵敏度测量，仅检测10.54 pg/ml的TXB2

2.高吞吐量格式，最多可在3小时内处理37个重复样本

3.广泛引用于同行评审出版物中的各种样本类型，包括粘膜、组织、血清、血浆、尿液等

4.日复一日、一批又一批可靠且可重复的结果

TXB2酶联免疫试剂盒文献参考：

NRF2 prevents hypertension, increased ADMA, microvascular oxidative stress, and dysfunction in mice with two weeks of ANG II infusion: C. Wang, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 314, R399 (2018), Application(s): ELISA of mouse urine, Abstract; Full Text

Effect of Regular Aerobic Activity in Young Healthy Athletes on Profile of Endothelial Function and Platelet Activity: K. Podgórska, et al.; BioMed Res. Int. 2017, 8715909 (2017), Application(s): Human plasma, Abstract; Full Text

Protective Effects of Total Flavones of Elaeagnus rhamnoides (L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats: Z. Wei, et al.; Evid. Based Complement. Alternat. Med. 2017, 8142562 (2017),

TXB2酶联免疫试剂盒相关研究：

TXB2 ELISA kit   ADI-901-002

PGE2 high sensitivity ELISA kit  ADI-930-001

PGE2 high sensitivity ELISA kit  ADI-931-001

LTB4 ELISA kit  ADI-900-068

LTB4 ELISA kit  ADI-901-068

PGE2 ELISA kit  ADI-900-001

来源：https://www.amyjet.com/products/ADI-900-002.shtml

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17β-雌二醇ELISA试剂盒，安全无放射性酶法测定

The 17β-Estradiol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.

Specific with low cross-reactivity to related steroids

Safe and non-radioactive enzymatic assay

Simple and color-coded reagents reduces error

Reliable and reproducible results day-after-day and lot-after-lot

Fully quantitative results that surpass semi-quantitative Western blot analysis

Alternative Name: Estrogen

Sensitivity: 28.5 pg/ml (range 29.3 - 30,000 pg/ml)

Assay Time: <3 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-900-174.

Wavelength: 405 nm

Species reactivity: Species independent

Shipping: Blue Ice Not Frozen

Long Term Storage: +4°C

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

Scientific Background: Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.

17β-雌二醇ELISA试剂盒是一种比色竞争酶免疫测定试剂盒，结果在3小时以内。在405 nm处读取吸光度。该试剂盒使用现成的液体色码试剂提供雌二醇的特异性和节省的非放射性定量，以减少误差。

17β-雌二醇ELISA试剂盒艾美捷特点：

1.对相关类固醇具有低交叉反应性的特异性

2.安全无放射性酶法测定

3.简单且颜色编码的试剂减少了错误

4.日复一日、一批又一批可靠且可重复的结果

5.超过半定量蛋白质印迹分析的全定量结果

17β-雌二醇ELISA试剂盒化学性质：

别名：雌激素

灵敏度：28.5 pg/ml（范围29.3-30000 pg/ml）

化验时间：<3小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种的培养上清液和唾液中的17β-雌二醇。血清和血浆使用ADI-900-174。

波长：405 nm

物种反应性：物种无关

运输：蓝冰未冻结

长期储存：+4°C

内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液3、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

17β-雌二醇ELISA试剂盒文献参考：

Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract;

Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants, Abstract; Full Text

A high level of TGF-B1 promotes endometriosis development via cell migration, adhesiveness, colonization, and invasiveness: U.K. Soni, et al.; Biol. Reprod. 100, 917 (2019), Abstract;

CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice: K. Zhang, et al.; J. Clin. Invest. 130, 2333 (2019), Abstract;

来源：https://www.amyjet.com/products/ADI-900-008.shtml

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化学参数说明丨艾美捷cGMP-ELISA试剂盒方案

This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.

Alternative size available: ADI-901-013 (5x96 wells)

Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.088 pmol/ml)

Rapid – reliable results in just 3 hours

Trusted – regularly cited in peer-reviewed publications

Reduce errors – ready-to-use color-coded reagents and pre-coated plates

Simple – efficient and well-established sample handling protocols

Reliable – consistent lot-to-lot performance

环GMP（cGMP）是由鸟苷酸环化酶对GTP的作用形成的，其水平通常比cAMP低10-100倍。一些激素，如乙酰胆碱、胰岛素和催产素，以及某些其他化学物质，如血清素和组胺，会导致cGMP水平升高。鸟苷酸环化酶的刺激剂，如血管扩张剂硝普钠、硝酸甘油、硝酸钠和一氧化氮（NO）也会刺激cGMP水平。

cGMP-ELISA试剂盒是一种用于定量测定细胞外cGMP的比色竞争免疫分析试剂盒。乙酰化可提高灵敏度（包括试剂）。在405 nm处读取吸光度。提供的即用液体色码试剂减少了误差。

cGMP-ELISA试剂盒艾美捷化学参数：

替代名称：循环GMP

灵敏度：（非乙酰化）0.37pmol/ml；（乙酰化）0.088pmol/ml（范围（非乙酰化）0.16-500pmol/ml；（乙酰化）0.16-100 pmol/ml）

化验时间：3小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种的培养上清液、血浆、血清、唾液和尿液中的cGMP。

波长：405 nm

物种反应性：物种无关

交叉反应性：环状GMP（100%）和<0.001%：GMP、GTP、环状AMP、AMP、ATP、环状UMP、CTP

使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。

运输：蓝冰运输

内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液2、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液、乙酸酐、三乙胺

cGMP-ELISA试剂盒——用于细胞外cGMP定量的灵敏（0.088pmol/ml）比色试剂盒。

cGMP-ELISA试剂盒艾美捷特点：

1.敏感–可选乙酰化方案将灵敏度提高10倍（0.088 pmol/ml）

2.仅需3小时即可获得快速可靠的结果

3.可信——定期在同行评审出版物中引用

4.减少错误–即用色码试剂和预涂板

5.简单、高效且成熟的样本处理协议

6.可靠–一致的批量性能

cGMP-ELISA试剂盒文献参考：

Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions: T.E. Sener, et al.; Int. J. Impot. Res. 30, 179 (2018), Application(s): Rat cavernosal tissue lysate, Abstract;

Guanylin and uroguanylin mRNA expression is increased following Roux-en-Y gastric bypass, but guanylins do not play a significant role in body weight regulation and glycemic control: M.L. Fernandez-Cachon, et al.; Peptides 101, 32 (2017), Abstract;

Sildenafil attenuates hypoxic pulmonary remodelling by inhibiting bone marrow progenitor cells: S. Favre, et al.; J. Cell. Mol. Med. 21, 871 (2017),

来源：https://www.amyjet.com/products/ADI-900-013.shtml

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艾美捷P物质ELISA试剂盒化学性质说明

The Substance P ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit utilized low sample volume and provides the ability to assay up to 38 samples in duplicate per 96 well kit.

Alternative size available: ADI-901-018A (5x96 wells)

Highly sensitive assay, measuring as little as 5.3 pg/ml of Substance P

Widely cited in numerous peer reviewed publications

High throughput fomat, assay up to 38 samples in duplicate per 96 well kit

Fully quantitative results that surpass semi-quantitative Western blot analysis

P物质ELISA试剂盒是一种比色竞争性酶免疫测定试剂盒，3小时内即可获得结果。在405 nm处读取吸光度。该试剂盒利用了低样本量，并提供了每96孔试剂盒最多38份样本的分析能力。

P物质ELISA试剂盒艾美捷化学性质：

灵敏度：5.3 pg/ml（范围9.76-10000 pg/ml）

化验时间：3小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种的培养上清液、血浆、血清、唾液和尿液中的P物质。引用的样本类型包括组织。

波长：405 nm

物种反应性：物种无关

使用/稳定性：在组件标签上标明的温度下储存组件。抗体、缀合物和标准品储存在-20°C，其余成分储存在4°C。为方便接收，包含所有组件的未开封试剂盒可在-20°C下储存，并在该温度下保持稳定，直到试剂盒到期。第一次解冻后，按照上述标签所示温度储存组件。应限制冻融循环，以获得特别的性能。

运输：蓝冰运输

内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

P物质ELISA试剂盒相关研究：

Substance P ELISA kit ADI-901-018A

Substance P  ALX-167-003

Substance P polyclonal antibody  BML-SA1270

Substance P (5'-TAMRA conjugate) BML-SX5800-0001

Substance P receptor NK1 polyclonal antibody  ADI-905-801-100

Absorbance 96 Plate Reader ENZ-INS-A96

P物质ELISA试剂盒ADI-901-018A

物质P ALX-167-003

P物质多克隆抗体BML-SA1270

P物质（5'-TAMRA缀合物）BML-SX5800-0001

P物质受体NK1多克隆抗体ADI-905-801-100

吸光度96读板器ENZ-INS-A96

来源：https://www.amyjet.com/products/ADI-900-018A.shtml

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艾美捷12(S)-HETE-ELISA试剂盒，比色竞争性测定

The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.

Easy-to-use color-coded reagents and pre-coated plates reduce errors and save time in the lab

High throughput format, obtain results from up to 39 samples in duplicate in just 5 hours

Highly sensitive assay, measure as little as 146 pg/ml

Reliable and reproducible results day-after-day and lot-after-lot

Sensitivity: 146 pg/ml (range 195 - 50,000 pg/ml)

Assay Time: 5 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue.

Wavelength: 405 nm

Species reactivity: Species independent

Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.

Shipping: Shipped on Blue Ice

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

12（S）-HETE对多形核白细胞和血管平滑肌细胞具有趋化性和化学动力学。它也作为血管紧张素II诱导的醛固酮产生的第二信使。

12(S)-HETE-ELISA试剂盒是一种比色竞争性酶免疫测定试剂盒，5小时内即可获得结果。在405 nm处读取吸光度。使用易于使用的液体色码试剂可获得快速、准确和可靠的结果，从而减少误差。

艾美捷12(S)-HETE-ELISA试剂盒——灵敏的ELISA试剂盒能够检测来自任何物种的培养上清液和血浆中的12（S）-HETE。

12(S)-HETE-ELISA试剂盒化学性质：

替代名称：12（S）-氢过氧四烯酸二十碳四烯酸

灵敏度：146 pg/ml（范围195-50000 pg/ml）

化验时间：5小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种培养上清液和血浆中的12（S）-HETE。引用的样本类型包括血清、肾间质液和组织。

波长：405 nm

物种反应性：物种无关

使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。

运输：蓝冰运输

内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

12(S)-HETE-ELISA试剂盒艾美捷特点：

1.易于使用的色码试剂和预涂板减少了错误并节省了实验室时间

2.高通量格式，仅需5小时即可获得多达39份样本的结果，一式两份

3.高灵敏度测定，测量值低至146 pg/ml

4.日复一日、一批又一批可靠且可重复的结果

12(S)-HETE-ELISA试剂盒文献参考：

12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of HETE levels in rats, Abstract; Full Text

Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016), Application(s): 12(S)-HETE assay, rabbit, Abstract; Full Text

12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015), Application(s): ELISA, Abstract; Full Text

Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015), Application(s): ELISA using MCF7 cell culture supernatants,

来源：https://www.amyjet.com/products/ADI-900-050.shtml

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艾美捷内皮素-1-ELISA试剂盒，2小时内可获得结果

The Endothelin-1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 2 hours. This ultra-sensitive kit lets you quantify as little as 0.41 pg/mL ET-1 in a variety of biological matrices with the option of overnight or a 1 hour primary incubation.

Highly sensitive measurement, quantify as little as 0.41 pg/ml ET-1, allowing you read both normal and pathological samples

Higher throughput format with results in just 2 hours for up to 38 samples in duplicate

Flexible option of overnight or 1 hour primary incubation

Suitable for a variety of sample matrices

Sensitivity: 0.41 pg/ml (range 0.78 - 100 pg/ml)

Assay Time: 2 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of ET-1 in culture supernatants, plasma, and serum of human, mouse, rat, cow, dog, pig, and rabbit origin. Cited sample types include cell lysates and tissue homogenates.

Wavelength: 450 nm

Species reactivity: Human, Mouse, Rat

Bovine, Dog, Porcine, Rabbit

Shipping: Blue Ice Not Frozen

Long Term Storage: +4°C

Contents: Microtiter plate, Antibody diluent, Antibody concentrate, Assay buffer 17, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2

内皮素-1-ELISA试剂盒——用于心血管和压力相关研究的灵敏、快速内皮素-1 ELISA试剂盒。它是一种比色免疫测定酶免疫测定试剂盒，2小时内即可获得结果。该超灵敏试剂盒可在多种生物基质中定量低至0.41 pg/mL ET-1，并可选择过夜或1小时初级培养。

内皮素-1-ELISA试剂盒艾美捷化学性质：

灵敏度：0.41 pg/ml（范围0.78-100 pg/ml）

化验时间：2小时

应用：ELISA，比色检测

应用说明：用于定量测定人、小鼠、大鼠、牛、狗、猪和兔来源的培养上清液、血浆和血清中的ET-1。引用的样品类型包括细胞裂解物和组织匀浆。

波长：450 nm

物种反应性：人、小鼠、大鼠

牛、狗、猪、兔

运输：蓝冰未冻结

长期储存：+4°C

内容：微量滴定板、抗体稀释剂、抗体浓缩液、测定缓冲液17、洗涤缓冲液浓缩液、标准品、TMB底物、停止溶液2

内皮素-1-ELISA试剂盒艾美捷特点：

1.高度灵敏的测量，可定量至0.41 pg/ml ET-1，允许您读取正常和病理样本

2.更高的吞吐量格式，仅需2小时即可处理多达38个重复样本

3.灵活选择过夜或1小时初级培养

4.适用于各种样本矩阵

内皮素-1-ELISA试剂盒文献参考：

miRNA-1 regulates endothelin-1 in diabetes: B. Feng, et al.; Life Sci. 98, 18 (2014), Application(s): ELISA using culture supernatants, ;

Serine Carboxypeptidase SCPEP1 and Cathepsin A Play Complementary Roles in Regulation of Vasoconstriction via Inactivation of Endothelin-1: X. Pan, et al.; PLoS Genet. 10, e1004146 (2014), Abstract;

Water deprivation induces neurovascular and cognitive dysfunction through vasopressin-induced oxidative stress: G. Faraco, et al.; J. Cereb. Blood Flow Metab. 34, 852 (2014), Application(s): ELISA using mouse tissue homogenates;

Acute effects of red wine on cytochrome P450 eicosanoids and blood pressure in men: A.E. Barden, et al.; J. Hypertens. 31, 2195 (2013), Application(s): ELISA

来源：https://www.amyjet.com/products/ADI-900-020A.shtml